Infrared and Raman Microscope Based on a Combination of Two Analytical Techniques to Provide Complementary Molecular Information.

This simple system improves the efficiency of analytical operations by making it easy to perform all process steps from sample observation to data analysis.

Examples of Problems Solved

 Contaminant components cannot be identified with an infrared microscope alone.

 The contaminant was identified based on both infrared and Raman measurement results. Measuring the same sample location with both infrared and Raman is desired.

 The infrared and Raman microscope enabled the same location to be quickly measured without moving the sample. The target area is too small to be measured with an infrared microscope.

 The infrared and Raman microscope enabled even smaller areas to be targeted and measured. Detailed analysis of both organic and inorganic components is desired.

 Combining infrared and Raman spectroscopy enabled material analysis.

Both FTIR and Raman Spectra Can Be Measured without Moving Samples

Both FTIR and Raman Spectra Can Be Measured without Moving Samples

Because samples do not need to be moved, both infrared and Raman spectra can be measured from the same position in an extremely small area. That means information about both organic and inorganic substances can be obtained from the same position, which can significantly improve the accuracy of qualitative analysis. In addition, Shimadzu’s proprietary wide-view camera and microscope camera (for infrared measurements) or objective lens (for Raman measurements) help improve sample observation efficiency. The wide-view camera not only enables observation of large areas up to 10 × 13 mm, but it also supports variable digital zooming. Furthermore, it shares positional information with the microscope camera and objective lenses.
The microscope camera can be used to observe areas as small as 30 × 40 μm, the 50× objective lens to observe areas as small as 15 × 20 μm, and the 100× objective lens to observe extremely small areas as small as 7.5 × 10 μm.

One Software to Measure and Analyze Both FTIR and Raman Spectra

One Software to Measure and  Analyze Both FTIR and Raman Spectra

You can easily switch between infrared and Raman measurements with a click. In addition, infrared and Raman spectra can be superimposed and displayed, and various analyses can be performed.

Obtain Organic and Inorganic Information with One Instrument

Obtain Organic and Inorganic Information with One Instrument

Infrared microscopes can analyze organic substances, but it is difficult to obtain information for many inorganic substances.
On the other hand, Raman microscopes can obtain information about inorganic substances such as titanium oxide and carbon, in addition to organic substances. In contrast, a single AIRsight unit can analyze mixtures of both organic and inorganic substances.

AIRsight Features for Raman Measurements

Confocal optical system used

Confocal optical system used
Enables Raman measurements with excellent spatial resolution

Equipped standard with 532 nm and 785 nm lasers
Characteristics of the lasers
532 nm : Light scatters more easily, making it easier to obtain peak intensities.
785 nm : Less affected by fluorescence, making it more suitable for fluorescent samples.

Systems can be equipped with either a 50× or 100× objective lens (or both)
Selectable depending on the target measurement area

Includes XYZ correction for lens switching between infrared and Raman measurements
Enables both infrared and Raman measurements from the same location.

2in1 solution

Length Measurement Function

  •   Infrared       Raman  

    AMsolution software now includes functionality for measuring lengths, including the lengths of objects in infrared Raman microscope images. Also, length measurement results can be output with a single button click.

Depth Measurements

  •   Raman  
    Enables Analysis in the Depth Direction (Z-Direction)

    Raman measurements can measure depth either at a single point or along a line*. If a transparent sample, such as plastic or glass, has a thickness (depth) dimension, the laser light components that can penetrate the sample can be used to measure the sample interior. Even if the sample is colored or cloudy, measurements are generally possible as long as the interior can be observed.

    * Measuring depth (at a line) requires using a separate mapping program (P/N 206-35093-41).


AMsolution includes measurement software (AMsolution Measurement) and analysis software (AMsolution Analysis). The measurement software can control both infrared and Raman measurements via the same window. That means all processes, from image acquisition to measuring infrared and Raman spectra at the same location can be performed smoothly. The analysis software can overlay and search infrared and Raman spectra, create libraries, and so on.
The data measured in infrared mode can be imported to LabSolutions IR and analyzed.*¹
The data measured with AIMsolution, a software program for infrared microscopes, can be analyzed with AMsolution, a software program for AIRsight.
*1 Only with LabSolutions IR Ver. 2.31 or later


Instrument Validation

A validation program is included as standard with the AMsolution measurement program for inspecting and validating the performance of Shimadzu infrared and Raman microscopes. The infrared mode is validated using a polystyrene film in accordance with the Japanese Pharmacopoeia, US Pharmacopeia, European Pharmacopoeia, and Chinese Pharmacopoeia. The Raman mode is validated using polystyrene pellets to inspect wavenumber accuracy in accordance with the Japanese Pharmacopoeia, US Pharmacopeia, and European Pharmacopoeia. That means analysts can inspect the basic performance of the instrument themselves to ensure that highly reliable data is obtained.

  • Infrared Mode Inspection Parameters
  • Raman Mode Inspection Parameters
    • Shape and size of the power spectrum
    • Based on the polystyrene film spectrum:
      – Resolution
      – Wavenumber accuracy
      – Wavenumber reproducibility
      – Transmittance (or absorbance) reproducibility
      – Peak resolution function
    • Note:
      ・ The wavenumber reproducibility is an inspection parameter only required for the Japanese Pharmacopoeia.
      ・ The Peak resolution function is an inspection parameter only required for the Chinese Pharmacopoeia.
      ・ The inspection parameter of US Pharmacopeia is only wavenumber accuracy.
    • Based on the polystyrene pellet spectrum:
      – Wavenumber accuracy

Library Creation Function

The functionality for creating libraries is included as standard with the AMsolution analysis program. Analysts can create their own library by registering the infrared and Raman spectra they acquire. Created libraries can also be used for searches. Registering the materials used in products and the substances used in manufacturing processes and using them as a library can improve the accuracy of searches.

Libraries Containing about 12,000 Spectra

​Systems are equipped standard with an extensive selection of libraries, including proprietary Shimadzu libraries and libraries for substances ranging from typical reagents to macromolecules. That means the standard configuration provides plenty of data for qualitative analysis without purchasing additional libraries.
​Main ContentsShimadzu Food Additives library
(substances listed in official analysis methods for food additives)
General reagentsPharmaceuticalsPesticidesPolymersInorganic substances

Total of about 12,000 spectra

Option Library

Wiley Raman spectrum libraries can be added. Wiley offers libraries of spectra from a wide range of compounds, including monomers, polymers, inorganic compounds, and compounds related to biochemistry or forensic chemistry.

Database example

Product NameDatabase CodeNumber of Spectra
Sadtler Controlled & Prescription Drugs 1-2RZ, RZ21,850
Sadtler Flavors & FragrancesFFR600
Sadtler InorganicsRI1,630
Sadtler Polymers & Monomers (Basic) 1QR1,680
Sadtler Polymers & Processing ChemicalsRA495
Sadtler Standards 1-6RST1, RST2, RST3,
KnowItAll Raman Spectral Library
(Annual Subscription)

Automatic Contaminant Recognition System

  • The functionality for automatically recognizing contaminants is included as standard. The analyst simply clicks one button for the software to automatically recognize contaminants. Two types are available for the infrared mode: the standard type or the micro type for extremely small areas, which can be selected based on the purpose of analysis. Samples can either be measured with the automatically selected measurement positions left unchanged or the analyst can add or delete measurement positions. A sample image is automatically saved for each measured spectrum. That makes it easy to confirm the sample or measurement positions later.


Please enter your comment!
Please enter your name here