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Pressure Cycling Technology (PCT)
Barocycler NEP 3229

Power of PCT

Pressure Cycling Technology (PCT) uses rapid cycles of hydrostatic pressure between ambient and ultra high levels to control biomolecular interactions, allowing for a high degree of precision, reproducibility, convenience, speed, and safety. This unique, patented technology offers the potential for broad applications in a number of established and emerging fields, including genomics, proteomics, drug discovery & development, process purification, pathogen inactivation, immunodiagnostics, and DNA sequencing.

The PCT Sample Preparation System (PCT SPS)

Sample preparation is a significant bottleneck to discoveries in genomic and proteomic research. To address this issue, the PCT SPS was developed, which allows for the safe, rapid, and reproducible extraction of DNA, RNA, small molecules, and proteins from a wide variety of cells and tissues, particularly those considered "hard-to-lyse". The PCT SPS uses an automated bench top instrument (Barocycler NEP3229), together with single-use sample processing containers (PULSETM Tubes).

The Barocycler NEP3229

The Barocycler NEP3229 is a lightweight, portable, and affordable instrument designed to fit on a laboratory bench top, inside a biological safety cabinet, or on the shelf of a cold room. The NEP3229 is capable of processing up to three samples simultaneously. The NEP3229 has an external chiller hook-up, automatic fill and dispense valves, and a microprocessor with an easy-to-use keypad. The Barocycler NEP3229 fills an important and growing need in the genomics, transcriptomics and proteomics sample preparation market for a small affordable instrument that provides safety, quality, versatility and reproducibility.

PULSE Tubes (PT)

PULSE Tubes (Pressure Used to Lyse Samples for Extraction) transmit the power of PCT from the Barocycler instruments to the sample. Specimens are placed on the Lysis Disk, the PT is placed in the pressure chamber, pressure chamber fluid is added, and pressurization begins. As pressure increases, the Ram pushes the sample from the Sample Chamber through the Lysis Disk and into the Fluid Retention Chamber. When pressure is released, the sample (now partially homogenized) is pulled back through the Lysis Disk by the receding Ram. The combination of physical passage through the Lysis Disk, rapid pressure changes, and other bio-physical mechanisms, breaks up the cellular structures quickly and efficiently, releasing the nucleic acids, small molecules, and proteins.

Features & Benefits of the PCT SPS

Safe
PULSE Tubes offer a closed system to reduce sample handling and minimize exposure to pathogens/toxins
Fast
Nucleic acids, small molecules, and proteins are released from a wide variety of cells and tissues in minutes
Powerful
Up to 35,000 PSI (235 MPa, 2.35 kbar) can be used to lyse samples and release excellent quality and quantity of bio-molecules
Efficient
Up to three samples can be extracted simultaneously, in minutes
Versatile
Animal, plant, and microbial samples can be processed; either standard or user defined protocols can be used
Reproducible
Computer controlled protocols mean consistent sample extraction each time, every time

 

 

 

 

 

   
 
 
 



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Bara Scientific Co., Ltd.
U Chu Liang Building Floor 7
968 Rama4 Road, Silom, Bangrak, Bangkok 10500 THAILAND
Tel ์ : 02-6324300 (auto 20 lines) Fax : 02-6375496-7

 

 

 
 
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