The most widely adopted and essential approach
to identifying, characterizing and comparing proteins is two-dimensional
gel electrophoresis separation followed by mass spectrometry
BioMachines’ 2D iD and 2D iDx systems automate the gel processing
steps, prior to identification by mass spectrometry. The 2D
iD is an integrated gel-imaging station and cutter, while
the 2D iDx has extended capabilities including in-gel digestion,
peptide extraction, and MALDI spotting.
The 2D iD and 2D iDx’s key differentiators include:
Broad span-of-solution in a single machine
Multi-function head and interchangeable tools that perform
eight different functions including gel plug cutting and ejection,
liquid handling with fixed and disposable tips, disposable
tip ejection, multiwell plate purging, multiwell plate transport
and washing on-the-fly using an integral wash station.
Quality of data results
Multi-wavelength transilluminator for imaging visible and
fluorescent stained gels with wavelength selection and intensity
under software control.
Reproducibility of data results
Ten (10) micron precision for cutting and dispensing with
+/- four (4) micron repeatability coupled with object detection
and automatic error recovery for monitoring and verifying
gel plug excision, transport and ejection.
Ease of integration with existing and planned software
Flexible 1D and 2D gel software for optimizing gel images,
for interactively generating cut lists, and for aligning images
and cutlists with data imported from many third-party image
Minimal supervision required
Walk-away gel processing in a single bench top system (2D
iDx) that performs gel imaging, in-gel digestion, peptide
extraction and MALDI target spotting.