The UV-2600i Plus is a single monochromator system with an expandable wavelength range up to 1400 nm using an optional integrating sphere, making it adaptable for various needs.
The UV-2700i Plus is a double monochromator system capable of 8-Abs measurements, ideal for low transmittance samples.

Both models come equipped with the intuitive Shimadzu LabSolutions UV-Vis software, ensuring data integrity and ease of use. Additionally, these spectrophotometers can be scheduled to shut down or enter sleep mode to save power and lamp life, and can automatically wake up at a specified time, enhancing efficiency. Elevate your analysis with these versatile and efficient spectrophotometers.

Perfect for a Wide Variety of Applications

The functionality of the UV-2600i Plus /2700i Plus can be freely expanded to suit the measurement objective. By accommodating a wealth of accessories, the system can address any user’s applications and a variety of situations. Thanks to intuitive operations, anyone can easily obtain the data required.

Single monochromator UV-2600i Plus

A key feature of the UV-2600i Plus equipped with a single monochromator is its measurement wavelength range. By using the optional ISR-2600Plus Integrating Sphere attachment, the measurement wavelength range can be extended from 220 nm to 1400 nm, significantly expanding its applications.

Integrating Sphere Enables Measurements to 1400 nm

The UV-2600i Plus is equipped with Shimadzu’s proprietary LO-RAY-LIGH grade diffraction grating, which achieves high efficiency and low stray light levels. By installing the ISR-2600Plus two-detector integrating sphere, the 300 nm to 1100 nm wavelength range of conventional models can be extended to 1400 nm. In addition, the UV-2600i achieves a significant noise reduction, and can accommodate measurements of solar cell anti-reflective films and polycrystalline silicon wafers.

Measures Slight Differences in Absorbance

Equipped with a double monochromator that achieves ultra-low stray light levels, the UV-2700i Plus is optimal for measuring low transmittance samples, such as polarization films used for LCD panels. The UV-2700i Plus is capable of 8 Abs measurements, and can make accurate transmittance measurements to 1 part in 100 million, accommodating a variety of sample measurements.

Achieves Ultra-Low Stray Light, Enabling 8-Abs Measurements

In the case of a device equipped with a general double monochromator, the absorbance that can be covered is about 5 to 6, but the UV-2700i Plus offers a range to 8 Abs, with a transmittance value of 0.000001 % (1 part in 100 million). This system achieves high-level absorbance measurements with incomparable precision. In addition to measuring even high-concentration samples as is, eliminating the need to dilute samples, the system can be applied to evaluating the transmission characteristics of polarization films. Wavelengths in the 400 nm to 650 nm range can be measured to 8 Abs.

Sample Polarization Film Measurement

With the rotating film holder (image below), two film samples can be set on the same optical axis. In this example, the polarization film is rotated in the plane, and the transmittance is measured when the film transmits and blocks light.

Enables Compliance with ER/ES Regulations and Stronger Data Management

Compatible with Validation from PC Software

Validation can be implemented with PC software using UV validation software. In addition to simplifying daily inspections, this makes instrument performance checks and records management easier, enabling more secure regulatory compliance.

Support for FDA 21 CFR Part 11, PIC/S GMP Guidelines and Other Regulations and Guidelines

 Ensuring the integrity of data (database management), including the user management, user authority management, and data audit trails required for compliance with FDA 21 CFR Part 11, PIC/S GMP guidelines, and other ER/ES regulations is possible.

Option Software Assists with Regulation-Compliant Validation

UV validation software assists with performing the measurements and calculations required for validation. The software is included standard with UV-2600i/2700i models. In addition to routine inspections, it can also be used to perform instrument validation that is compliant with Japanese, US, and European pharmacopoeias.

What is Instrument Validation?

Instrument validation is an essential process for assessing and managing instrument health. What are the key elements of validation and how should they be performed? For more details, refer to the explanation page.

Assist Function

The instrument assists the user to ensure that measurements
are performed using the correct procedure.^*1

*1 This function can be enabled/disabled.

Shutdown/Wakeup Functions

The instrument can be shut down, and put into sleep mode. Putting the instrument into sleep mode limits power consumption and helps preserve the lamp. After a long period of measurements, the instrument and software can automatically be shut down. After shutdown, the instrument can also be set to wake up automatically at a specified time. With this function, analysis can start the instant the user arrives at the laboratory.

SolidSpec, TMSPC, LabSolutions, the Analytical Intelligence logo, LO-RAY-LIGH and PPSQ are trademarks of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.
Excel, Microsoft and Azure are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries.
Amazon Web Services and AWS are trademarks of Amazon.com, Inc. or its subsidiaries.
Google Cloud Platform and GCP are trademarks of Google LLC.

The UV-1900i Plus is a high-performance double-beam UV-Vis spectrophotometer with Shimadzu’s LO-RAY-LIGH diffraction grating technology. It offers 1 nm resolution, low stray light and noise, and ultra-fast scanning at 29,000 nm/min. The user-friendly multi-language interface supports various measurement modes, including photometric, spectrum, quantitation, kinetics, time-course, and bio-methods. It ensures high-accuracy quantitative analysis, detects low-concentration components, and complies with Pharmacopeia standards (JP, USP, EP).

With low stray light levels and high reproducibility, the UV-1900i Plus is ideal for accurate analysis of both low and high concentrations. It supports network connectivity for remote data checks (with additional memory) and can operate as a stand-alone unit with a color touch panel or via PC using LabSolutions UV-Vis software. Enhanced by a new CPU, it offers faster response speeds, efficient analysis, and integrated data management.

Refined User-Friendliness

Easy-to-Use Interface
Grasp the Current Status and Operating Procedures at a Glance

The UV-1900i Plus on-screen user interface includes large, easy-to-see icons deployed on a black background, so the instrument settings are evident at a glance. In addition, the large, easy-to-see icons improve intuitive understanding, which enables users to quickly become familiar with the operations. Furthermore, the user interface is designed to minimize transitions between windows, so users do not get confused during the operations. The system also features a faster response speed by adopting a new CPU.

Easy-to-Use Interface
Grasp the Current Status and Operating Procedures at a Glance

The UV-1900i Plus on-screen user interface includes large, easy-to-see icons deployed on a black background, so the instrument settings are evident at a glance. In addition, the large, easy-to-see icons improve intuitive understanding, which enables users to quickly become familiar with the operations. Furthermore, the user interface is designed to minimize transitions between windows, so users do not get confused during the operations. The system also features a faster response speed by adopting a new CPU.

Multiple Measurement Modes

UV-1900i Plus has six basic measurement modes, which offer the optimal solution for a variety of measurements.

Photometric

Measures the photometric value at a single wavelength or multiple (up to eight) wavelengths.
 

Spectrum

Measures a sample spectrum using wavelength scanning.

 

Quantitation

Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns.

Kinetics

Measures absorbance changes as a function of time and obtains the enzymatic activity values. The kinetics measurement method or the rate measurement method can be selected

• Time CourseMeasures changes over time in photometric values at a specified wavelength.

• BiomethodQuantifies DNA or protein concentrations.

* The background color of the operation screen can also be set to white.

Various Functions for Comfortable Daily Measurement

• Assist Function  The instrument assists the user to ensure that measurements
  are performed using the correct procedure.^*1
  • 

Checks whether or not baseline correction, auto zero correction or cell blank correction has been performed and informs the user if it has not been.

Informs the user if the last correction performed is not appropriate for the planned measurement

• Informs the user if the UV-1900i Plus is not ready to begin measurement and when starting measurements
  and 100 %T (0 Abs) corrections.

*1 This function can be enabled/disabled.

Shutdown Function

The shutdown function puts UV-1900i Plus to sleep after it’s been used, or after a certain period of time. Putting the instrument into sleep mode limits power consumption and helps preserve the lamp.

Wakeup Function

Automatically wakes up a sleeping UV-1900i Plus at a specified time. This function eliminates the need for analysts to wait for it to warm up, allowing them to be more productive.

Startup Validation Function

Instrument performance checks can be performed automatically　when the instrument is turned ON.^*2
This enables even more reliable instrument operation.
It can also be implemented in combination with the Wakeup function.

*2 This function can be enabled/disabled.
The performance check items that can be used with this function are as follows: wavelength accuracy (D2), wavelength repeat accuracy (D2), degradation, noise level, baseline flatness, baseline stability (drift), [EP] Control of Wavelength Accuracy (D2), and [USP] Control of Wavelengths (D2).

Network Connectivity Function

Data can now be transferred to a PC via a network. With wireless printing, multiple UV units can print from a single printer.^*3 (A router and other network equipment must be installed to use a network.)

*3 A router with a wireless function is required.
*4 Optional expanded memory is required. The instrument is not compatible with control via a network.
*5 A PictBridge-compatible printer is required.

Bar-Code Reader and Keyboard Entry Function

Sample names and numerical values can be entered by a bar-code reader or from the keyboard.
This saves time when entering sample names for a multiple-sample analysis, and prevents sample misidentification and other human errors.^*6

*6 Use a bar-code reader and keyboard with a USB connection.

High Performance to Meet Diverse Needs

Ultra-Fast Scan

Spectra can be acquired as fast as 29,000 nm/min. Ultra-fast scanning is effective in tracking chemical reactions in a short time. In addition to the absorbance change at specified wavelengths, spectra can also be acquired in a short time with the UV-1900i Plus. Therefore, more detailed behavior can be investigated by observing spectra with the UV-1900i Plus.
The figures below show the analysis of the particle agglomeration process when salts are added to silver nanoparticles. Measurements of the 300 to 700 nm region were performed in ultra-fast scan mode. In addition to the decrease of absorbance at 400 nm and the increase of absorbance at 480 nm, the temporal changes of spectra can also be observed.

Low Stray Light

Linearity will be lost in the high absorbance region due to stray light. UV-1900i Plus is equipped with Low Stray Light Diffraction Gratings, achieve “best-in-class” low stray light. Stray light is at 0.5 % max. (198 nm), making accurate measurements possible at 1 Abs or more, even in the ultraviolet region. In addition, high-concentration samples can be quantified accurately.
The figure on the right is a calibration curve for acetic acid, created with absorbance at 200 nm. The correlation coefficient is 0.9998 and correct measured values are obtained even with high-concentration samples.

High Reproducibility and Repeatability Accuracy

The photometric repeatability accuracy is 0.0002 Abs max. (0.5 Abs and 1.0 Abs). As a result, variance in the measurement results is suppressed, enabling more accurate quantitation and the detection of low-concentration samples.
The figure on the right is a calibration curve for caffeine, created with absorbance at 273 nm.
The calibration curve has an Abs = 0.0528 Conc. The lower limit of quantitation determined from the standard deviation is 0.0051 mg/L.

Note: One method of determining the lower limit of quantitation is to use ten times the standard deviation. This is an actual measured value and is not guaranteed.

Validation Functions Compliant with JP, USP, and EP (Touch-screen Display)

This instrument can not only run checks for nine JIS items, but also those stipulated in the Japanese Pharmacopoeia (JP), United States Pharmacopeia (USP), and the European Pharmacopoeia (EP). The UV-1900 series also complies with the hardware specifications required by each Pharmacopoeia. In addition, the check conditions can be saved. As a result, checks can be performed easily just by calling them up as needed. Check results can also be saved.

Improved Security Functions

An external control security function has been added to provide more support for compliance with regulations. Three user authority levels, “Administrator”, “Developer”, and “Operator”, can be set for instrument users.

Resolution of 1 nm, the Highest in its Class

In addition to achieving a resolution of 1 nm, the highest in its class, by using a monochromator with a Czerny-Turner mounting, the UV-1900i Plus also features a compact, bright optical system. The instrument is more than capable of meeting the wavelength resolution required in the USP and EP.

Compatible with Validation from PC Software

UV validation software (limited function edition) is included as standard. Using this software, inspections can be implemented as stipulated for 9 items in JIS and the JP. If optional UV validation software (USP/EP compatible edition) is added, examinations can be implemented as stipulated in the USP and the EP.

Support for FDA 21 CFR Part 11, PIC/S GMP Guidelines and Other Regulations and Guidelines

Ensuring the integrity of data (database management), including the user management, user authority management, and data audit trails required for compliance with FDA 21 CFR Part 11, PIC/S GMP guidelines, and other ER/ES regulations is possible.

Reliable LabSolutions Software

 In addition to LabSolutions UV-Vis, which provides basic functionality, Shimadzu also offers LabSolutions DB UV-Vis and LabSolutions CS UV-Vis to meet the requirements of ER/ES regulations.

This simple system improves the efficiency of analytical operations by making it easy to perform all process steps from sample observation to data analysis.

Examples of Problems Solved

 Contaminant components cannot be identified with an infrared microscope alone.

 The contaminant was identified based on both infrared and Raman measurement results. Measuring the same sample location with both infrared and Raman is desired.

 The infrared and Raman microscope enabled the same location to be quickly measured without moving the sample. The target area is too small to be measured with an infrared microscope.

 The infrared and Raman microscope enabled even smaller areas to be targeted and measured. Detailed analysis of both organic and inorganic components is desired.

 Combining infrared and Raman spectroscopy enabled material analysis.

Both FTIR and Raman Spectra Can Be Measured without Moving Samples

Because samples do not need to be moved, both infrared and Raman spectra can be measured from the same position in an extremely small area. That means information about both organic and inorganic substances can be obtained from the same position, which can significantly improve the accuracy of qualitative analysis. In addition, Shimadzu’s proprietary wide-view camera and microscope camera (for infrared measurements) or objective lens (for Raman measurements) help improve sample observation efficiency. The wide-view camera not only enables observation of large areas up to 10 × 13 mm, but it also supports variable digital zooming. Furthermore, it shares positional information with the microscope camera and objective lenses.
The microscope camera can be used to observe areas as small as 30 × 40 μm, the 50× objective lens to observe areas as small as 15 × 20 μm, and the 100× objective lens to observe extremely small areas as small as 7.5 × 10 μm.

One Software to Measure and Analyze Both FTIR and Raman Spectra

You can easily switch between infrared and Raman measurements with a click. In addition, infrared and Raman spectra can be superimposed and displayed, and various analyses can be performed.

Obtain Organic and Inorganic Information with One Instrument

Infrared microscopes can analyze organic substances, but it is difficult to obtain information for many inorganic substances.
On the other hand, Raman microscopes can obtain information about inorganic substances such as titanium oxide and carbon, in addition to organic substances. In contrast, a single AIRsight unit can analyze mixtures of both organic and inorganic substances.

AIRsight Features for Raman Measurements

Confocal optical system used
Enables Raman measurements with excellent spatial resolution

Equipped standard with 532 nm and 785 nm lasers
Characteristics of the lasers
532 nm : Light scatters more easily, making it easier to obtain peak intensities.
785 nm : Less affected by fluorescence, making it more suitable for fluorescent samples.

Systems can be equipped with either a 50× or 100× objective lens (or both)
Selectable depending on the target measurement area

Includes XYZ correction for lens switching between infrared and Raman measurements
Enables both infrared and Raman measurements from the same location.

Length Measurement Function

•   Infrared       Raman  
  
  AMsolution software now includes functionality for measuring lengths, including the lengths of objects in infrared Raman microscope images. Also, length measurement results can be output with a single button click.

Depth Measurements

•   Raman  
  Enables Analysis in the Depth Direction (Z-Direction)
  
  Raman measurements can measure depth either at a single point or along a line*. If a transparent sample, such as plastic or glass, has a thickness (depth) dimension, the laser light components that can penetrate the sample can be used to measure the sample interior. Even if the sample is colored or cloudy, measurements are generally possible as long as the interior can be observed.
  
  * Measuring depth (at a line) requires using a separate mapping program (P/N 206-35093-41).

AMsolution

AMsolution includes measurement software (AMsolution Measurement) and analysis software (AMsolution Analysis). The measurement software can control both infrared and Raman measurements via the same window. That means all processes, from image acquisition to measuring infrared and Raman spectra at the same location can be performed smoothly. The analysis software can overlay and search infrared and Raman spectra, create libraries, and so on.
The data measured in infrared mode can be imported to LabSolutions IR and analyzed.*¹
The data measured with AIMsolution, a software program for infrared microscopes, can be analyzed with AMsolution, a software program for AIRsight.
*1 Only with LabSolutions IR Ver. 2.31 or later
 

Instrument Validation

A validation program is included as standard with the AMsolution measurement program for inspecting and validating the performance of Shimadzu infrared and Raman microscopes. The infrared mode is validated using a polystyrene film in accordance with the Japanese Pharmacopoeia, US Pharmacopeia, European Pharmacopoeia, and Chinese Pharmacopoeia. The Raman mode is validated using polystyrene pellets to inspect wavenumber accuracy in accordance with the Japanese Pharmacopoeia, US Pharmacopeia, and European Pharmacopoeia. That means analysts can inspect the basic performance of the instrument themselves to ensure that highly reliable data is obtained.

• Infrared Mode Inspection Parameters
• Raman Mode Inspection Parameters

• • Shape and size of the power spectrum
  • Based on the polystyrene film spectrum:
    – Resolution
    – Wavenumber accuracy
    – Wavenumber reproducibility
    – Transmittance (or absorbance) reproducibility
    – Peak resolution function
     
  • Note:
    ・ The wavenumber reproducibility is an inspection parameter only required for the Japanese Pharmacopoeia.
    ・ The Peak resolution function is an inspection parameter only required for the Chinese Pharmacopoeia.
    ・ The inspection parameter of US Pharmacopeia is only wavenumber accuracy.
• • Based on the polystyrene pellet spectrum:
    – Wavenumber accuracy

Library Creation Function

The functionality for creating libraries is included as standard with the AMsolution analysis program. Analysts can create their own library by registering the infrared and Raman spectra they acquire. Created libraries can also be used for searches. Registering the materials used in products and the substances used in manufacturing processes and using them as a library can improve the accuracy of searches.
 

Libraries Containing about 12,000 Spectra

​Systems are equipped standard with an extensive selection of libraries, including proprietary Shimadzu libraries and libraries for substances ranging from typical reagents to macromolecules. That means the standard configuration provides plenty of data for qualitative analysis without purchasing additional libraries.
​Main ContentsShimadzu Food Additives library
（substances listed in official analysis methods for food additives）General reagentsPharmaceuticalsPesticidesPolymersInorganic substances

Total of about 12,000 spectra

Option Library

Wiley Raman spectrum libraries can be added. Wiley offers libraries of spectra from a wide range of compounds, including monomers, polymers, inorganic compounds, and compounds related to biochemistry or forensic chemistry.

Database example

Automatic Contaminant Recognition System

• The functionality for automatically recognizing contaminants is included as standard. The analyst simply clicks one button for the software to automatically recognize contaminants. Two types are available for the infrared mode: the standard type or the micro type for extremely small areas, which can be selected based on the purpose of analysis. Samples can either be measured with the automatically selected measurement positions left unchanged or the analyst can add or delete measurement positions. A sample image is automatically saved for each measured spectrum. That makes it easy to confirm the sample or measurement positions later.

An automatic analysis system that can be used with confidence from your first analysis
Equipped as standard with enhanced functionality to support analyses

AIMsight, the Analytical Intelligence logo, LabSolutions, IRXross, and IRTracer are trademarks of Shimadzu Corporation or its affiliated companies in Japan and/or other countries. 

Observe

Loading Samples Is Easy

Pressing the [Eject Sample] button makes it easy to load and remove samples by automatically lowering the stage and switching the objectives to expand space. Furthermore, the lower Condenser mirror can be removed to enable reflectance/ATR measurements of samples up to 40 mm thick.

Look for the Item to be Measured
-Quickly Determine the Measurement Position-

Wide-Field Camera and Microscope Camera

The wide-field camera and microscope camera help observe samples efficiently. In addition to observing a large area up to 10 × 13 mm, the wide-field camera also supports variable digital zooming. Furthermore, by sharing positional information with the microscope camera, it achieves a digital zoom function capable of zooming to a magnification of about 330× for observing areas as small as 30 × 40 µm. (The microscope camera supports variable digital zoom magnifications up to 10×.)

Perform Infrared Measurements Smoothly While Viewing Visible Images
– Visible/Infrared Dual View System –

Infrared spectra can be measured while checking a visible image of the sample. Spectra can be measured while confirming the position of objects, which avoids the trouble of switching back and forth between visible light and infrared light. Used in combination with the tiling function, visible observations, and infrared measurements can be performed anywhere within the stage operating range, eliminating the need to reposition the sample.

Image view of the visible light and infrared optical path
          Green light: visible light optical path
          Red light : infrared optical path         

Measure

Automatic Contaminant Recognition System

• The functionality for automatically recognizing objects is included standard. The analyst simply clicks one button for the software to automatically recognize objects. Two types are available: the standard type or the micro type for extremely small areas, which can be selected based on the purpose of analysis. Samples can either be measured with the automatically selected measurement positions left unchanged or the analyst can add or delete measurement positions. A sample image is automatically saved for each measured spectrum. That makes it easy to confirm the sample or measurement positions later.

Perform Ultra Micro Analysis  – Best-in-Class S/N Ratio-

The AIMsight has been optimized to measure extremely small areas. The AIMsight achieves a 30,000:1 S/N ratio, the best in its class. Consequently, it can quickly obtain excellent spectra from even microscopic objects.

Transmission measurement of polystyrene beads

A ø10 μm polystyrene bead was measured by transmission method. Low noise and a high-quality spectrum of a very small sample were obtained with only a small number of scans.

High-Sensitivity ATR Measurement  – Samples with High Refractive Index –

Due to a steep incident angle for the infrared light, the AIMsight is able to acquire excellent distortion-free ATR spectra even when measuring samples with a high refractive index, such as black rubber. In addition, the Ge prism (P/N 206-32600-41) has an anti-reflective coating that provides high sensitivity.

ATR measurement of black rubber
An acrylonitrilebutadiene rubber (NBR) with 50 wt% carbon was measured with a 
Ge-ATR objective. A clear peak of C=C-H out-of-plane bending mode was obtained at 970 cm^-1, which was strained with a gradual incident angle.                                                   

Length Measurement Function

A length measurement function has been added to AMsolution. The length of the target object can now be measured in images obtained with the infrared microscope.
In addition, with a single click, the length measurement results can be output as a report.

Analyze

Fully Supports the Identification of Contaminants

Various libraries are available, such as Contaminant Library, Thermally-Damaged and UV-Damaged Plastic Library.

• Contaminant Library for LabSolutions IR
• Thermally-Damaged Plastics Library
• UV-Damaged Plastics Library

Automatic Identification of Object
-Contaminant Analysis Program-

The contaminant analysis program – the functionality for automatically qualifying objects – is included as a standard feature in LabSolutions IR software. Measured spectra using AMsolution can be loaded directly into LabSolutions IR and analyzed. The contaminant analysis program identifies measured objects with high precision using a spectral library for substances commonly detected as contaminants in combination with Shimadzu’s proprietary identification algorithm (patent JP5205918). Even if objects are mixtures, the system searches for the primary and secondary components and displays the probability of the substances identified. It is not necessary to specify the number of components in the mixture, so analysis is easy, even for people with little experience with infrared analysis.

Spectrum Advisor Function (patent pending)

This is packaged with operations specific to infrared microscopes and advice on troubleshooting using accessories. Corrective measures are proposed by comparing the measured spectrum with measurement examples. As a result, better-quality data can be acquired.

Visualize Components- Chemical Imaging* –

The invisible distribution of chemicals can be visualized based on peak height or area, multivariate analysis (PCR/MCR), or spectral similarity to target spectra.

Chemical image of pharmaceutical powder

Pharmaceutical products were rolled with a diamond cell, then mapping measurement was performed. The figure to the right represents the distribution of powder components, such as lactose, lipid, and cellulose. The color display can be switched freely between single-color and multi-color.
 

* Visualizing chemicals requires an optional mapping program (P/N 206-35093-41).

Tm analysis systems can accelerate the development process and improve the quality of oligonucleotide therapeutics. Control by LabSolutions^TM software enables compliance with ER/ES-related regulatory requirements and improves the efficiency of analyzing the thermal stability (Tm analysis) of nucleic acids.

What is Tm Analysis System

Tm analysis serves an important role in checking the thermal stability and sequence of nucleic acids. In Tm analysis, heat is applied to the nucleic acids in double-strands. Then, the change in absorbance (melting curve) that occurs as the temperature increases and the strands dissociate into single strands is measured. The melting temperature (Tm value) is determined as the temperature where the mole fractions of single and double strands are equal.

LabSolutions, TMSPC and the Analytical Intelligence logo are trademarks of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.
Excel is either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries.

Reliable Data Integrity

System Configuration

Tm analysis system consists of the following.

• UV-Vis Spectrophotometer
• TMSPC^TM-8i 8-cell thermoelectrically temperature controlled cell holder
• LabSolutions UV-Vis Tm software

Data Integrity

Tm analysis systems comprise a spectrophotometer, TMSPC-8i 8-cell thermoelectrically temperature-controlled cell holder, and LabSolutions UV-Vis Tm software. They can automatically determine Tm values based on melting curve measurements using the average method or derivative method. LabSolutions UV-Vis Tm can provide the industry’s highest data integrity levels when linked to a LabSolutions DB/CS system, which has an extensive track record from use with LC, GC, and many other analytical instruments.

Note: LabSolutions DB UV-Vis: This is a stand-alone database management system that analysis software on a single PC.
           LabSolutions CS system: A server, client, and acquisition controller are connected on the network. The data of all devices are centrally managed by the database built on the server. User and permission management is centrally managed with this system.

Functionality for Satisfying Various Needs

8-Cell Micro Multi-Cell

Melting Curve of Nucleic Acid (M13 Primer)
(Using Micro Multi-Cell with 1 mm Optical Path Length) 

This micro multi-cell can be used to measure eight samples at the same time using the same measurement conditions.
In addition to previous micro multi-cell models with a 10 mm optical path length (with a 100 μL minimum sample volume) for satisfying high-sensitivity measurement needs, a new model with a 1 mm optical path length (with a 10 μL minimum sample volume) for measuring micro-quantities of expensive samples has been added to the lineup. Cells with a 1 mm optical path length can result in sample evaporation problems, but a new sealing method*, which suppresses the evaporation of samples by sealing the top side of the cell, enables reliable measurement of samples with high melting temperatures.

*The sealing method was supported by Professor Junji Kawakami (Department of Nanobiochemistry, Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan University, Japan). This was supported by AMED under Grant Number JP21ae0121022, JP21ae0121023, JP21ae0121024 (Project leader: Satoshi Obika).

Real-Time Transfer Function and Thermodynamic Parameter Analysis

LabSolutions UV-Vis Tm can transfer temperature, absorbance, and time values to an Excel® file in real-time during measurements.
By transferring Tm values obtained from samples with different concentration levels to commercial spreadsheet software, changes in Gibbs free energy values used as an index for drug activity levels or thermodynamic properties, such as entropy or enthalpy, can be analyzed easily.

Efficient Automated Workflow

Conventional Tm analysis requires multiple steps, including checking the UV-Vis spectrum, annealing, measuring the melting curve, and analyzing data, with recording and data management performed separately. In the past, the spectrum measurement, annealing, and melting curve data were discrete. LabSolutions UV -Vis Tm software supports the entire process from measurement to analysis (Tm value calculation) with a menu that is easy to understand, even for those new to Tm analysis. It achieves a seamless workflow and efficient Tm analysis by performing time-consuming annealing, correction (background wavelength and temperature blank correction), and Tm value calculation (average or derivative methods) steps automatically. In addition, measurement data and analysis values are saved as a set, and various settings and operation histories are managed by an Audit Trail Log.

Measuring a sample spectrum

Set the sample and measure the spectrum.

■ Spectrum data and the melting curve can be easily confirmed by switching tabs

Automate annealing with simple settings

By raising and lowering the temperature of the sample, the nucleic acids are made single-stranded and then re-bonded to double-stranded. This reduces the number of binding mismatches, enabling stable analysis.

■ Automated annealing with temperature program setting
■ Automated flow reduces the risk of sample mix-ups during the transition between measurement and annealing.
■ Automatic recording of annealing and temperature settings

Easy setup and automatic Tm measurement and analysis

It measures the change in absorbance during the melting process of nucleic acids from double strands to single strands as the temperature rises.
The Tm value (the melting temperature at which the mole fractions of double-stranded and single-stranded molecules are equal) is calculated by the average method, which determines a value based on the intersection of the melting curve and the median line of the tangents of the sections selected in the pre and post-transition region, or by the derivative method obtained from the maximum value of the first derivative.

■ Tm analysis supports both average and derivative methods

■ All application logs from measurement to analysis are automatically recorded to ensure reproducibility

■ Automatic calculation of Tm values enables analysis that eliminates the need for human intervention

Example of Tm Analysis

This system can measure the number of photons absorbed, which is necessary for calculating the quantum yield of photoreactions.

Photoreaction Quantum Yield:

For a comparison to data obtained with the conventional method using a chemical actinometer, refer to LAAN-A-UV-E041 Application News bulletin No. A478.

• Application News No. A478 “QYM-01 Photoreaction Quantum Yield Evaluation System”

This photoreaction evaluation system was developed by Shimadzu Corporation, under the supervision of Professor Osamu Ishitani and Associate Professor Yusuke Tamaki of the Department of Chemistry, Graduate School of Science and Engineering, Tokyo Institute of Technology.
Analytical Intelligence logo, PQY and Lightway are trademarks of Shimadzu Corporation.

Shortens Experimental Process

Conventional measurement requires a lengthy process using a chemical actinometer but Lightway includes functionality that calibrates the number of irradiated photons, eliminating this process.

*Using a chemical actinometer involves sample adjustment process steps, such as crystallizing, air drying, and diluting samples, and then measuring the number of photons in a dark room and calibrating the number of photons irradiated on the sample.

Measuring is Easy

It can monitor spectral changes* during the photoreaction process in real time.
Measurements can be started by specifying mainly three parameter settings
(measurement interval, measurement time, and number of irradiated photons),
which makes it easy to operate even for first-time users. Also, a dialog box is
displayed whenever a button is pressed to help navigate operations.

* Results for specific wavelengths can be graphed as a function of time.

Irradiated light settings
LEDs provide stable, bright, and long-lasting irradiation light. That means reliable results can be obtained anytime. Also, experimental reproducibility can be increased by specifying the quantity of light irradiated.
 

Time-course measurement results at a monitored wavelength
Time-course changes in the absorbance at any particular wavelength can be determined instantaneously. By specifying the specic wavelength as the monitored wavelength before starting a  measurement, spectral changes can be displayed as a function of time, so that the end of the reaction can be conrmed more quickly and the reaction rate can be conrmed more intuitively.

Measurement results for all spectra
No bothersome settings are necessary. Only the measurement interval and measurement time need to be specied for spectra measurement conditions. The minimum measurement interval setting is 0.1 seconds. That means spectra from 250 to 800 nm can be acquired within 0.1 seconds to track reactions quickly.

The PQY-Meas software that comes standard with the system offers the following three modes.

Photoreaction Measurements

Measures a spectrum while the sample is irradiated by the irradiation light source.

Spectral Measurements

Measures a spectrum after the sample is irradiated by the irradiation light source. It can also perform regular spectral measurements as a spectrophotometer.

Calibration Curve & Quantitation

Creates a calibration curve from a standard sample to determine concentrations in unknown samples.

Measures Photon Count Accurately

Previously, a chemical actinometer was required to calculate the number of irradiated photons, but there were concerns about variability caused by the chemical actinometer adjustment process. The Lightway system uses a power meter to calibrate the number of irradiated photons and make corrections based on spectral shape prior to measuring, which prevents variability caused by differences in skill levels.
    It also features a stable and long-lasting LED irradiation light source. 

Using an LED irradiation light source means reliable measurements can be obtained over long periods.

The figure above illustrates one example of how light source intensity decreases over time. The red line indicates the intensity of an LED irradiation light source, whereas the blue line indicates the intensity of a xenon lamp irradiation light source. It shows that the LED irradiation light source offers high intensity for a longer time and the intensity decrease is more gradual than for the xenon lamp irradiation light source.

An optional LED irradiation light source is available.*

* This product is manufactured by CELL System Co., Ltd. Therefore, it must be purchased as an optional product.

The LED irradiation light source can be replaced easily.

High Sensitivity

Relationship between Detectors and Measurable Range

The photomultiplier tube detector can be switched to the InGaAs detector in the range from 700 nm to 1000 nm (the default switching wavelength is 870 nm). The InGaAs detector can be switched to a PbS detector in the range from 1600 nm to 1800 nm (the default switching wavelength is 1650 nm).

High accuracy for transmittance and reflectance is required for the measurement of optical parts. The SolidSpec-3700i/3700i DUV have three detectors which cover the range from ultraviolet to near-infrared. The sensitivity in the near-infrared region is significantly enhanced by using both InGaAs and cooled PbS detectors. Highly accurate and highly sensitive spectra are obtainable from ultraviolet to near-infrared.High accuracy for transmittance and reflectance is required for the measurement of optical parts. The SolidSpec-3700i/3700i DUV have three detectors which cover the range from ultraviolet to near-infrared. The sensitivity in the near-infrared region is significantly enhanced by using both InGaAs and cooled PbS detectors. Highly accurate and highly sensitive spectra are obtainable from ultraviolet to near-infrared.

Conventional spectrophotometers have used a photomultiplier tube detector for the ultraviolet and visible region and a PbS detector for the near-infrared region. However, neither detector has much sensitivity near the detector switchover, preventing high sensitivity measurement in this range. The SolidSpec-3700i/3700i DUV make it possible to take high sensitivity measurements in the switchover range by using an InGaAs detector as shown in the figure on the left.

Wide Measurement Wavelength Range (SolidSpec-3700i DUV)

The development of precise laser machining using an ultraviolet laser such as an ArF excimer laser enhances the requirement for transmittance or reflectance measurements of optical parts in the deep ultraviolet region. The SolidSpec-3700i DUV^(note1) enables measurement in the range of 175 nm to 2600 nm^(note2) with an integrating sphere and the range of 165 nm to 3300 nm^(note3) by mounting the optional Direct Detection Unit DUV. With this additional unit, the wide range from deep ultraviolet to near-infrared is now measurable.

Oxygen molecules in the atmosphere absorb ultraviolet light under 190 nm. Nitrogen gas purging for both the optical and the sample compartment is required to remove the interfering oxygen molecules. Since the SolidSpec-3700i DUV has purge inlets for each compartment, efficient nitrogen gas purge is possible so that the time required for purging after sample replacement is reduced, and high sensitivity with lower stray light in the deep UV region is achieved.

Materials which do not absorb deep ultraviolet light are required to be used as the window material for the detector and the material for the inside of the integrating sphere to enable performance in the deep ultraviolet region. The SolidSpec-3700i DUV uses a PMT detector with fused silica as the window material and an integrating sphere with resin that has highly reflective characteristics in the deep ultraviolet region as the inside material.

Note1) In order to measure the range below 190 nm with the SolidSpec-3700i DUV, nitrogen gas purge is required to remove interference from oxygen molecules inside the SolidSpec-3700i DUV.
Note2) The measurable range for SolidSpec-3700i is 240 nm to 2600 nm.
Note3) The measurable range for SolidSpec-3700i with the optional Direct Detection Unit is 190 nm to 3300 nm.

• Related Application

Large Sample Compartment Accommodates a Wide Variety of Samples.

The SolidSpec-3700i & 3700i DUV have large sample compartments which allow large samples to be measured without sample destruction. Their internal dimensions are 900W × 700D × 350H mm. A sample maximum of 700W × 560D × 40H mm can be set in the sample compartment and an entire sample area of 12 inches or 310 × 310 mm is measurable by mounting the Automatic X-Y stage (option). The vertical optical path makes it possible to perform transmission or reflectance measurements of large samples.

Large Sample Compartment
A sample of 700W × 560D mm is set in the sample compartment.

Three Dimensional Optical Path

The three-dimensional optical path enables non-destructive measurement of large samples, without having to cut them smaller. In the optical path of previous models, light only traveled horizontally, but the new models include a three-dimensional optical path (U.S. patent 6583872) with light also traveling in the vertical direction. Samples can be placed horizontally, which makes it easier to place large samples.

A wide variety of accessories, such as absolute specular reflectance attachments and relative specular reflectance attachments created for the SolidSpec-3700i/3700i DUV; expand the application range. Automatic measurements can be performed with the optional Automatic X-Y Stage by inputting the intervals and the rotation angles for the sample.

Automatic Measurement

Automatic X-Y Stage (Option)

The Automatic X-Y Stage developed for the SolidSpec-3700i/3700i DUV enables automatic measurements for the points specified in advance while maintaining the nitrogen gas purge.

Direct Measurement of Liquid Samples and Solid Samples without Integrating Sphere

Direct Detection Unit (Option)

The SolidSpec-3700i/3700i DUV measure samples with an integrating sphere as the standard detection system. However, some samples require measurement without using an integrating sphere. The Direct Detection Unit was created for just such instances. By mounting the Direct Detection Unit (DDU-DUV) in the SolidSpec-3700i DUV, measurements down to 165nm^(note) are possible. Measurements with the Direct cDetection Unit can be performed simply by switching a mirror.

Note) In order to measure below 190 nm with SolidSpec-3700i DUV, nitrogen gas purging is required to remove interfering oxygen molecules inside of the SolidSpec-3700i DUV.The measurable range for the SolidSpec-3700i with the optional Direct Detection Unit is 190 nm to 3300 nm.

Features

BioSpec-nano

The BioSpec-nano is a spectrophotometer suitable for carrying out concentration checks for DNA and RNA nucleic acid samples. Quantitation of DNA and RNA can be rapidly and simply carried out with very small samples ranging from 1µL, and analysis throughput is improved due to dedicated functions, such as automated sample mounting and wiping, and easy-to-use operation software

All operations from sample mounting to measurement and wiping are automatically performed. Tedious operations are unnecessary.

Quick Analysis

Just drop the sample onto the target and click the button. That’s all there is to analysis. The instrument will perform sample mounting, measurement, and wiping. No need to move the wiper arm manually, nor wipe the liquid-contact parts with a cloth. Spectra measurement is conducted and the analysis data is obtained quickly.

1 µL – 2 µL Nucleic Acid Quantitaion *

Analysis can be performed with 1 µL (pathlength: 0.2 mm) or 2 µL (pathlength: 0.7 mm) samples. The sample is dropped onto the target with a pipette. The optional 5 mm cell allows measurement of 5 mm path length. * If the surface tension of the sample is small, droplets might not be formed with a 1 to 2 µL sample.

Easy-to-use Software

Basic operations can be conveniently performed by clicking icons in the software or function keys on the instrument itself. Analysis results can be converted to PDF or CSV files.