To keep pace with evolving laboratory needs, Shimadzu continually enhances its high-performance analytical systems.

The new LCMS-8065XE is a triple-quadrupole mass spectrometer with EVOLVED, EFFICIENT, and EXACT capabilities. These exceptional capabilities ensure high reliability and enhanced productivity, empowering the laboratory for the future.

Reflecting our core philosophy of “Contributing to Society Through Science and Technology”, Shimadzu is committed to addressing current and future challenges by continually advancing the hardware and software of its analytical instruments—delivering meaningful value to society through innovation.

EVOLVED

Revolutionary New Technology Achieves Exceptional Performance

Our latest technologies included in the LCMS-8065XE bring sample analysis to a new level. Through evolution of our UF technologies, ESI probe, and collision cell, we are pushing the boundaries to achieve the highest level of performance.

Exploring the Core of High-Sensitivity Analysis

The newly designed StreamFocus ESI probe enhances UFsensitivity analysis by reducing droplet dispersion within the ESI spray. Equipped with a specially engineered low-diffusion nebulizer nozzle, it enables greater ion intake while reducing nebulizer gas consumption—making it particularly well-suited for reducing operational expenses.

The IonFocus unit in the LCMS-8065XE optimizes ion transmission. It solves the common trade-off between contamination reduction and sensitivity loss by using an electrode to focus and direct only target ions into the mass spectrometer, excluding neutral contaminants. This results in high sensitivity, exceptional robustness, and enhanced instrument uptime for reliable, long-term analysis.

EFFICIENT

New UF Technologies Enable Ultra-Fast Mass Spectrometry Applications

Shimadzu’s triple quadrupole mass spectrometers, powered by a suite of proprietary UF Technologies, have earned widespread acclaim from users across diverse applications. The new LCMS-8065XE incorporates the latest advancements in these technologies, delivering unmatched sensitivity and faster analysis speeds—redefining what’s possible in quantitative mass spectrometry.

UF Technology (Learn more)

UFsweeper Ⅳ

Shimadzu’s proprietary UFsweeper technology—designed to efficiently guide ions through the collision cell without energy loss—has been further refined. The updated collision cell features a larger entrance lens diameter and an increased number of lenses, significantly boosting ion throughput. These improvements result in enhanced ion transmission and greater sensitivity, enabling more precise and reliable quantitative analysis.

Easy Maintenance Shortens Instrument Downtime

Both the desolvation line (DL), which delivers samples to the vacuum section, and the ESI capillary can be replaced quickly and easily. Even the DL can be replaced without breaking vacuum, helping to minimize downtime and maintain laboratory productivity.

Maximizing Overall Workflow Efficiency

• Intelligent tools to help your workflow efficiency generating higher productivity and better data quality.
• 

Preparation

Instrument Check 

PERFORMANCE CONCIERGE makes tuning a mass spectrometer easier than ever before. A tuning standard is automatically introduced into the instrument to verify parameters, including mass accuracy, resolution, and signal strength. Based on these checks, tuning may be automatically initiated to ensure optimal performance. Should the criteria not be met, PERFORMANCE CONCIERGE will diagnose the issue and alert the operator of required maintenance and maximize uptime.

Optimization of Acquisition Conditions

Automates MRM optimization to create high sensitivity quantitative LC-MS/MS methods. LabSolutions Connect MRM not only optimizes target component MRM transitions and collision energies but also maximizes the response for on column analysis. All optimized acquisition parameters are stored in a data base helping to accelerate both method development and routine analysis.

LabSolutions Connect (Learn more) 

Startup 

Integrated intelligence on the Nexera LC series that allows you to increase column life-times and reduce consumables by enabling automated column equilibration for flow rates and temperature.

FlowPilot (Learn more)

Reviewing Data and Reporting Results

LabSolutions Insight supports a dynamic quantitative data review landscape with the tools to drive high confidence in result reporting by ‘review by exception’. Insight can be configured to check for any outliers outside the scope of user defined criteria for reporting results.

LabSolutions Insight (Learn more)

Data Analysis 

Peak Integration 

Peakintelligence for LCMS (Learn more)

• 
• This example demonstrates the use of Peakintelligence for integration of LC/MS peaks of per- and polyfluoroalkyl substances (PFAS). It confirms accurate peak integration even for signals with low signal-to-noise (S/N) ratios.　Peak intelligence for LCMS also reduces detection errors caused by baseline noise or undulations to achieve more consistent quantitative analysis results. Revolutionary AI-driven peak detection and integration technology delivers consistent, accurate results even for challenging low signal-to-noise peaks. The intelligent algorithm automatically identifies components and integrates peaks without requiring manual parameter adjustment, while effectively suppressing false positives caused by baseline noise and drift.

• 
• PeakIntelligence dramatically reduces time-consuming manual review processes (up to 67%) and delivers superior reproducibility across complex analyses like PFAS quantification. Experience higher confidence in your results with automated processing that maintains accuracy while accelerating your analytical workflow.

EXACT

The chromatogram below illustrates the part-per-trillion analysis of a 1 ng/L mixed standard solution of PFAS (Per- and Polyfluoroalkyl Substances). Despite the extremely low concentration of 1 ng/L, the compounds are detected with good peak intensity. The LCMS-8065XE, equipped with the innovative StreamFocus and UFsweeper IV, delivers exceptional sensitivity for the analysis of trace-level components.

The LCMS-8065XE exhibits exceptional robustness during continuous analysis of complex matrices. The data above show both absolute peak areas relative to the initial response and peak area ratios normalized using internal standards, obtained from over 10,000 consecutive injections of treated wastewater samples. To create a more challenging analytical environment, matrix elimination using LC switching valves was deliberately omitted. Even under these challenging conditions, the system retained over 85% of its initial response after 10,000 injections, highlighting its exceptional durability and stability in demanding high-throughput applications.

Championing Sustainability for a Better Tomorrow

High Energy Efficiency

The LCMS-8065XE includes ecology mode functionality that checks the system usage status and automatically shuts down the system if it has not been used for a certain period of time. Using the ecology mode can reduce electricity consumption by about 31%. It also contributes significantly to reducing power consumption by not using LC-MS/MS peripheral equipment except during analysis. Moreover, reducing CO₂ emissions contributes to achieving a carbon-neutral society.

Fast-Tracking Sustainability with Nexera UHPLC and LCMS-8065XE

• Combining the LCMS-8065XE with a Nexera series UHPLC system enables ultra-fast analysis and significantly enhances analytical throughput. This increased efficiency also leads to substantial reductions in solvent, gas, and electricity consumption, resulting in lower operational costs.
• NOTE: Power consumption calculations assume 8 hours of analysis on weekdays and no hours on weekends (with ESI interface switched OFF)
  
  Without Ecology Mode
  ESI interface remains ON after analysis.
  
  With Ecology Mode
  Ecology mode is activated after analysis (with ESI interface switched OFF).

UFMS, Analytical Intelligence logo, UFsweeper, UFsensitivity, IonFocus, UF-Qarray, UF-Lens, UFscanning, UFswitching, UF-MRM, PERFORMANCE CONCIERGE, LabSolutions Connect, LabSolutions Insight, LabSolutions, Peakintelligence and Nexera are trademarks of Shimadzu Corporation or its af filiated companies in Japan and/or other countries.

Radicalize Your Mass Spectrometer to Solve Unanswered Questions

The OAD-TOF system is a Q-TOF LCMS that realizes OAD (Oxygen Attachment Dissociation), Shimadzu’s proprietary fragmentation technology. It allows the analysis of the position of carbon-carbon double bonds in lipids and other organic compounds. Combined with the LCMS-9050, which boasts world-class high mass accuracy and fast polarity switching, it enables the creation of innovative applications.

Innovative fragmentation technology

The OAD-TOF system is equipped with an OAD RADICAL SOURCE I, which enables Oxygen Attachment Dissociation (OAD) analysis, an innovative fragmentation that utilizes Shimadzu’s proprietary radical reaction. It can measure fragment ions that cannot be obtained by conventional collision-induced dissociation (CID), where ions are fragmented by collision with an inert gas such as argon or nitrogen. The irradiating oxygen radicals specifically oxidize/dissociate double bonds between carbons, which is useful for structure estimation of organic compounds such as lipids. The technique can be applied to monovalent ions and negative ions, which have been difficult to fragment using radical reactions by electrons and anions, and provides completely new structural information

What is OAD (oxygen attachment dissociation)

CID preferentially dissociates weak chemical bonds. In the case of lipids, CID can determine the basic structure of lipids and polar groups, and the carbon composition of side chains (number of carbons and double bonds). With OAD, on the other hand, oxygen radicals react specifically with the double bonds between carbons, causing dissociation. By measuring fragment ions specific to the double bond, the position of the double bond can be determined.

Innovative technologies to advance LCMS-9050

The LCMS-9050, renowned for its exceptional stability in mass accuracy and high-speed polarity switching, pairs perfectly with the OAD-TOF system. This combination allows for innovative applications and surpasses the known boundaries of mass spectrometry.

LCMS-9050

• Radical source
  Neutral radicals such as O/OH/H radicals are generated by microwave discharge of raw material gases (water vapor and hydrogen gas) in a vacuum. The neutral radicals are introduced into the OAD cell through a quartz tube.
• OAD cell
  Precursor ions are reacted with neutral radicals generated in a radical source and fragmented. A collision gas (argon) can be introduced, and conventional CID fragmentation is also possible by setting the collision energy (CE).
• UF-FlightTube
  Precise temperature control is achieved due to an optimized heater and heater sensor layout and a robust control system. This minimizes the effects of any room temperature variations to enable stable mass accuracy over long periods. It also reduces the time and trouble required for mass calibration.
• iRef TOF
  The unique electrode shape achieves an ideal electric potential distribution that increases energy-focusing, while also inhibiting trajectory divergence and flight time lengthening during ion reflection.LCMS-9050

Surprisingly simple

Easy to switch between CID and OAD

CID is also possible with the OAD RADICAL SOURCE I installed. No special software is required, and LabSolutions LCMS control and analysis software for LCMS-9050 can easily switch between OAD and CID for analysis. Acquisition of a large amount of structural information by OAD and CID can lead to new discoveries in lipids and other organic compounds.

Reliable structure estimation with high mass accuracy

The high mass accuracy achieved with the LCMS-9050 remains unchanged even with OAD. High-quality MS/MS data within ±3 ppm enables reliable structure estimation of compounds, including determination of the position of carbon-carbon double bonds.

Creating endless possibilities

The OAD-TOF system can be used in combination with various options compatible with the LCMS-9050. The DPiMS QT, Kit for Direct Probe Ionization Mass Spectrometer, can be installed in place of the standard ESI unit for rapid analysis with simple pretreatment. It can also be connected to the Nexera UC supercritical fluid chromatography (SFC) system. This is especially useful for profiling lipids, where vast numbers of isomers exist.

DPiMS
Kit for Direct Probe Ionization Mass Spectrometer

DPiMS QT

Lipids in butter were analyzed using DPiMS in combination with the OAD-TOF system. Specific fragment ions derived from OAD were observed with only a simple pretreatment, which is a feature of DPiMS, and the double bond position of triacylglycerol was quickly estimated.

Nexera UC
Supercritical Fluid Extraction (SFE)-
Supercritical Fluid Chromatography (SFC)

Nexera UC

α-linolenic acid and γ-linolenic acid with different double bond positions were analyzed using Nexera UC and the OAD-TOF system. Isomers with similar structures were completely separated by SFC and detected with specific fragment ions derived from OAD, enabling highly accurate analysis. OAD can also be adapted to monovalent negative ions such as fatty acids.

iRefTOF, UF-FlightTube, LabSolutions, DPiMS and Nexera are trademarks of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.

Ultrafast Track to Your Success

• Innovative technology, exceptional design and new ways of thinking are part of our engineering DNA delivering solutions for the ever-changing needs of any laboratory. As our scientific and business needs change our engineering design evolves and adapts. The result is the RX Series of triple quadrupole LC-MS instruments designed with unmatched capability, redefined reliability and creating a new standard in actionable data.

LCMS-TQ RX Series Line-Up

Reliable

LCMS-TQ RX Series CoreSpray Technology 

1, CoreSpray

CoreSpray

Enhanced Ionization Performance

CoreSpray, a newly developed gas delivery system, improves performance and uniformity of nebulization with higher flows and better heat transfer.

• 2, Heated ESI ProbeThe RX Series are equipped with a heat-assisted electrospray ionization probe. The resulting highly efficient desolvation process promotes the ionization of a wide range of target compounds. 
• 3, IonFocus UnitAll models are also equipped with Shimadzu’s unique IonFocus unit. The focusing electrode efficiently delivers ions into the mass spectrometer.

Ultrafast Sensitivity and Performance 

Shimadzu’s RX Series expands the capability and productivity of triple quadrupole mass spectrometry with performance that can be relied upon. The RX Series has been designed to generate a highly focused ion beam from the ion source to detector bringing together advanced ion guide and collision cell technologies. The result is higher data quality that makes an impact on any LC-MS/MS assay. 
UF Technology  (Learn more) 

Peak Area Stability in Matrix: 20,000 Continuous Injections

CoreSpray technology applied to the repeated analysis of pesticides in black tea matrix resulting in enhanced robustness (%RSD less than 3.5%, 20,000 injections). Data generated without internal standards and without diverting to waste for the initial polar matrix effects.

Resilient

Maximizing Overall Workflow Efficiency

• Intelligent tools to help your workflow efficiency generating higher productivity and better data quality.
•  

Preparation

Instrument Check 

PERFORMANCE CONCIERGE makes tuning a mass spectrometer easier than ever before. A tuning standard is automatically introduced into the instrument to verify parameters, including mass accuracy, resolution, and signal strength. Based on these checks, tuning may be automatically initiated to ensure optimal performance. Should the criteria not be met, PERFORMANCE CONCIERGE will diagnose the issue and alert the operator of required maintenance and maximize uptime.

Optimization of Acquisition Conditions

Automates MRM optimization to create high sensitivity quantitative LC-MS/MS methods. LabSolutions Connect MRM not only optimizes target component MRM transitions and collision energies but also maximizes the response for on column analysis. All optimized acquisition parameters are stored in a data base helping to accelerate both method development and routine analysis.LabSolutions Connect (Learn more) 

Startup 

Integrated intelligence on the Nexera LC series that allows you to increase column life-times and reduce consumables by enabling automated column equilibration for flow rates and temperature.FlowPilot (Learn more)

Data Analysis 

Peak Integration 

Peakintelligence for LCMS is a world-first AI driven peak integration algorithm requiring zero user settings. The impact is dramatic. Peakintelligence for LCMS delivers higher consistency in peak integration and results in higher data quality and with far less time manually reviewing data.Peakintelligence for LCMS (Learn more)

• PeakIntelligence for LCMS was applied to the analysis of a panel of pesticides in a residue screening study highlighting consistent peak integration at low S/N or with overlapping components.

• PeakIntelligence for LCMS is an AI driven algorithm for component detection and peak integration without the need for any user parameter. PeakIntelligence for LCMS processing results in higher consistency in peak integration and reduces the time-consuming impact of manual review to achieve a more accurate, more reproducible peak processing.

Reviewing Data and Reporting Results

LabSolutions Insight supports a dynamic quantitative data review landscape with the tools to drive high confidence in result reporting by ‘review by exception’. Insight can be configured to check for any outliers outside the scope of user defined criteria for reporting results.LabSolutions Insight (Learn more)

Maintenance

Easy Maintenance Shortens Instrument Downtime

The RX Series keeps the same user-friendly maintenance as its predecessors. Changing the ESI capillary and the desolvation line (DL), which transports ions into the vacuum region, are designed for quick and effortless replacement without breaking vacuum helping to reduce time spent on maintenance and increase instrument uptime.

Responsible

Minimizing Global Environmental Impact

Highly Energy Efficient

The LCMS-TQ RX Series prioritizes environmental responsibility with its built-in ecology mode. This mode monitors system usage and automatically shuts down the instrument when idle for extended periods, reducing electricity consumption by an impressive 31%. Additionally, powering down peripheral LC-MS/MS equipment during non-analysis periods further minimizes energy use. These features contribute significantly to lowering operating costs and CO₂ emissions while supporting the pursuit of a carbon-neutral future.

Reduced Waste to the Environment

The new oil-free addition to our rotary pump line eliminates the labor of oil changes, reduces overall oil consumption, and produces less environmental waste. Additionally, PERFORMANCE CONCIERGE and ecology mode minimize solvent and power consumption, reducing environmental impact.

UFMS, IonFocus, UF-Qarray, UF-Lens, UFsweeper, UFscanning, UF-MRM, UFswitching, PERFORMANCE CONCIERGE, Analytical Intelligence logo, LabSolutions Connect, Nexera, Peakintelligence and LabSolutions Insight are trademarks of Shimadzu Corporation or its affiliated companies in Japan and/or other countries.

Stable performance eliminates differences between analysts, providing peace of mind

Shimadzu developed the MUP-3100 Fully Automated Sample Preparation Module for Glycan Analysis in conjunction with Sumitomo Bakelite Co., Ltd., for use with their antibody N-glycan analysis kit. The MUP-3100 eliminates manual sample preparation, increases overall analysis throughput, and improves reproducibility.

Auto-EZGlyco is a trademark of SUMITOMO BAKELITE CO., LTD. and/or its affiliates.

Precisely Automated Protocols Eliminate Human Error

Pretreatment is required for LC analysis of profiles for antibody-derived glycans, including purification of the antibodies, glycan cut-out, and fluorescent labeling. The MUP-3100 Fully Automated Sample Preparation Module for Glycan Analysis is equipped with functions to perform dispensing, the addition of reagents, heating, centrifugation, solid phase column extraction, and sample delivery, thereby automating the entire pretreatment sequence.

Pretreatment of antibody-derived glycans in culture supernatants involves repeated operations including dispensing, the addition of reagents, centrifugation, and heating. With solid phase column extraction using an antibody trap carrier, rinsing must be performed multiple times, which is labor intensive. The MUP-3100 reliably implements pretreatment using the Auto-EZGlyco mAb-N Kit for SHIMADZU protocol. This eliminates human errors, such as forgetting to add the reagent, taking the wrong sample, or omitting a process.

Simply Place the Sample and Reagents in the Instrument to Automatically Implement Pretreatment

Simply place the prepared reagent and the culture supernatants or other samples into the prescribed place for the instrument and select  pretreatment to implement the process automatically, quickly and accurately. The MUP-3100 allows for setting a maximum of 24 samples at one time and enables processing twice a day. The MUP-3100 not only lessens the burden on the analyst, but also enables pretreatment at night or on holidays, thereby accelerating the workflow.

Automatic Confirmation of Consumables Positioning Supports Stable Operations

With the MUP-3100, mistakes are prevented by checking the sample and necessary reagents positioning via image processing prior to automated operations. The automated protocol will be paused if the sample, reagent, or consumables are missing. Even during automated operations, pipette tip attachment/detachment and the column grip are monitored by sensors, preventing malfunctions.

Antibody N-glycan analysis kit “Auto-EZGlyco^TM mAb-N Kit for SHIMADZU”

This is capable of everything from purifying antibodies from unpurified samples (e.g. cell culture solution) and other partially purified samples, to glycan cut-out, fluorescent labeling of glycans and glycan purification. The kit also includes necessary reagents such as a ta glycan release enzyme and a glycan fluorescent labeling reagent.

Refer to Sumitomo Bakelite Co., Ltd.Auto-EZGlyco

Quality Control Support Functions

The control software of the MUP-3100 automatically generates pretreatment results reports and stores them in the database along with pretreatment conditions. The MUP-3100 continuously monitors its operating performance, and if the device detects an error, its internal camera automatically creates a video of the 5 seconds before and after the occurrence, which is then saved along with error logs. This assists in identifying the root cause of errors and minimizing downtime.

Processes 24 Culture Supernatant Samples Reliably in 6 Hours

Conventional methods require complex operations to cut out glycans from antibody samples in the culture supernatant. The traditional method requires more than two days for preprocessing. When using the MUP-3100 and the antibody N-glycan analysis kit in combination, however, 24 samples can be preprocessed in six hours. Glycan profiling, including the type and quantity of glycans, can be performed by analysis of 2-AB labeled glycans using an HPLC fluorescence detector. The Shim-pack GIST-HP Amide [Metal Free] column is effective for separating out impurities and glycans contained in antibody preparations and culture supernatants.

The MUP-3100 can process 24 samples at a time. Even with simultaneous processing of other samples, the area ratio of each peak remains stable regardless of peak intensity, yielding data equivalent to that obtained when using the kit manually.

Visualization of Temporal Change for Component Variations in Culture Media

The C2MAP System can be automated the process from pretreatment to measurement for culture supernatant samples and display temporal changes in the components as graphs. With the optimized LC/MS/MS simultaneous analysis method, the system can be used for the optimization of culture conditions in animal cell cultures by monitoring the consumption and depletion of media components during culturing, as well as the variation in metabolites secreted from cells.

Features of the C2MAP System

1. Automated Process from Pretreatment to Measurement for the Culture Supernatant Analysis

• The automated process allows analysis to be performed at night and on non-working days.
• The measurement workflow can be selected to match the actual culture.
• Seamless analysis and management from pretreatment to LC/MS/MS measurement can be achieved.
• Pretreated samples are stocked on a microplate automatically to enable re-measurement with ease.

2. Supports a Wide Range of Measurement Compounds and Culture Supernatant Samples

• A total of 95 components, including major basal culture media components for animal cells and secreted metabolites, can be simultaneously analyzed at high speed.
• Applicable to a wide range of cell culture media
  (iPS cells, ES cells, mesenchymal stem cells, T cells, and CHO cells)

3. Visualization of Component Variations in Culture Media

• Temporal changes in the components can be displayed as trend graphs.
• The results under multiple experimental conditions can be overlaid in the display, enabling comparative analysis.

Modules Required for Automated Pretreatment Can Be Operated Independently of LC/MS/MS

• The LC/MS/MS can be installed in a different room, allowing to be shared for other analyses.
• If culture samples cannot be taken out of the culture room, sample pretreatment can be performed in the culture room and measured by LC/MS/MS in another room.
• Separating the various components saves laboratory space.

C2MAP TRENDS

For results obtained via LC/MS/MS, temporal changes in each component can be graphed by the dedicated viewer software. Analysts can monitor variations in metabolites secreted from cells and culture media components during cultivation, as well as display graphs of component comparisons with samples from different culture series. As a result, the consumption and depletion of culture media components, and changes in the amounts of metabolic components secreted from cells, can be observed, thereby providing useful insights about the optimal culture conditions and assessments of cellular status.

Temporal Changes in Measured Components

Measured-component Comparisons among Different Culture Series

CLAM-2040

The CLAM-2040 accompanies you on most of the analytical workflow and improves your overall throughput by drastically reducing the sample preparation time. Simply place the blood tubes in position and the CLAM-2040 performs the next steps automatically, from sample extraction, up to the LCMS analysis and data processing.

Effortless performance

Sample extraction and analysis are fully automated.

The CLAM-2040 performs it all automatically: dispensing the sample onto the specialized filter vial, adding reagents, shaking, heating, filtering and transferring the extracted sample to the LCMS system autosampler.

Easy to use and to maintain. Reduced workload for operators.

Containers for liquid waste and filter waste reduce the infection risk and ensure operator safety. Easy access to all system automated parts, consumables, and waste also allows for easy maintenance over time.

An intuitive, user-oriented design for greater comfort in the work routine.

Intuitive software

• Easy to pilot with no need for expertise nor training,
• Easy to optimize and modify existing methods,
• Easy to parameter your own methods.

Easy reagent management

• Reagent vials of 3 different volumes for maximum adaptability and flexibility : 1.5 mL, 6 mL,or 12 mL,
• Free configuration of reagents (commercial, LDT),
• Easy to configure your own methods.
• Clear display of available reagents and remaining usage count,
• Automatic detection of missing reagent before starting analysis.

No technical knowledge is required to operate the system

Easy Data Review

• Easy visualization of calibration results,
• Easy visualization of Quality Control results,
• Data alerts when results are out of specification,
• Easy review of sample quantitative results,
• Direct communication with LIS for data reporting.

Easy to pilot, with straightforward steps, for rapid analysis starting.

Simply scan your sample ID using the barcode reader, select the analysis method, and press start. The CLAM-2040 will perform the next steps automatically.

Standardized workflow

Sample preparation overlap: one result every 2.6 min in an optimized workflow.

The latest quality control functionalities, including alerts.
Reduces variability in sample results, thanks to the absence of manual steps.
Prevents operator errors during the sample preparation procedure.
Lowers the risk of infections, improving the safety of work place safety.

Operational productivity

Improved overall efficiency : higher throughput at a lower cost.

The CLAM-2040 and LCMS instrument accompanies you on most of the analytical workflow and improves your overall throughput by drastically reducing the sample preparation time. Additionally, the CLAM-2040 has the capability to prepare up to 3 samples simultaneously to further optimize your routine. While the manual or semi-automated sample extraction will deliver one analysis result every 12 to 60 min, the CLAM-2040 and LCMS, in optimized conditions, will deliver up to one analysis result every 2.6 min.

Automation enables LCMS analysis to be performed any time, any day.

Run samples at night and weekends. Add samples and consumables continuously with the option to submit priority analysis requests for urgent samples.

No compromise. High efficiency and high comfort.

Improved efficiency. Higher throughput at a lower cost by reducing manual operations. Open access system with no need for intensive user training (meaning no additional cost).

LCMS technology enables multiplex analysis.

Associated with the CLAM-2040, LCMS technology enables you to analyze several compounds simultaneously, leading to time saving and overall cost reduction.

Capability to connect to your laboratory information system (LIS).

Bidirectional communication (HL7) with your laboratory information system (LIS) enables to submit analysis request from the LIS and to receive result report in LIS after analysis.

Higher user comfort.

Decrease of manual operation makes the workflow more comfortable for the user. Controlled safety creates peace of mind, and the intuitive software makes it effortless to run samples.

Flexibility

CLAM-2040 is the unique flexible LCMS automate.

Shimadzu has always attached great importance to providing the best solutions, while maintaining the highest possible flexibility for the operator, and providing products of great robustness. These key advantages are at the heart of our developments in all our products. Here too, the CLAM-2040 comes with high flexibility and gives reliable results, maintaining confidence over time thanks to its high robustness.

Use any application of your choice.

• Compatible with a wide range of Shimadzu methods,
• Supports Shimadzu databases for toxicology (TQ or high-resolution Q-TOF),
• Supports Shimadzu Metabolites Method Package Suite, Alsachim reagents can be used,
• Third-party reagents can also be used,
• Also supports your own laboratory developed tests.

Freedom to change.

• Methods can be optimized and modified,
• New methods can be freely added, with no need for Shimadzu intervention,
• Creating your homebrew method (LDT) is possible at any time.

One automate, unlimited possibilities.

• Freedom to choose your LCMS configuration and to evolve it (see hereafter details),
• Additional purifications available, such as online solid phase extraction (SPE),
• Various samples and reagents containers available to meet the diversity of routine needs,
• The LC-MS/MS system can also be used independently.

 From the blood tube, the CLAM can perform the extraction and LCMS analysis, automatically.

The LCMS system can also be used independently, at any time.

Full evolution potential

Free to evolve your LCMS technology at any time.

To meet your required sensitivity, you are free to choose your LC-MS/MS system by selecting one of our triple quadrupoles, from LCMS-8040 (not shown below) to LCMS-8060NX. Your needs evolve? You can change your MS system at any time. For your most exigent screenings, high resolution is also at your reach with our time-of-flight systems (QTOF). These options keep your flexibility at maximum.

Freedom to choose your LCMS TQ configuration and to upgrade it in the future.

High resolution LCMS is also available (Q-TOF), facilitating large screenings.

Innovation

Create a laboratory ready for tomorrow’s transitions with no human intervention between the conveyor and the result.

Lead the way to the future of the clinical research, be a pioneer.

The LCMS-9050 is a quadrupole Time-of-Flight (Q-TOF) mass spectrometer system designed with technologies developed for Shimadzu LCMS series models and that results in among the highest mass accuracy stability levels available. Shimadzu’s proprietary high precision temperature control system inhibits even tiny mass variations caused by external factors, so that the system can be used to measure accurate mass values without worrying about mass calibration. The stable positive/negative high-speed polarity switching technology enables the simultaneous analysis of positive ions/negative ions, contributing to new applications development and heightened analysis efficiency. To ensure that all users can perform analysis easily and efficiently, Shimadzu has aimed to simplify mass calibration, maintenance and other non-analysis procedures. Further, combining this system with Shimadzu’s renowned lineup of varied optional equipment enables greater utilization of the characteristics of Q-TOF while greatly expanding the range of data obtainable.

Simpler Accurate Mass Spectrometry

Technologies inherited from the Shimadzu LCMS series

The LCMS-9050 provides excellent performance thanks to Shimadzu’s proprietary patented technology inherited from the LCMS series. The system is equipped with technology mainly inherited from the LCMS-8000 series, from the injection unit to the ion separator. Ions are efficiently injected into the TOF unit thanks to the bright ion optical system and high ion convergence. At the TOF unit, the precision temperature control system inhibits mass accuracy variations caused by external factors. The heater and temperature sensor are arranged at the optimal flight tube positions, providing highly accurate and robust temperature control. In addition, the electrical system controlling the flight tube is temperature controlled to achieve high mass stability.

Stable long-term mass accuracy

The precision-controlled temperature control system minimizes the effects of room temperature variations, so the LCMS-9050 provides extremely stable mass accuracy. For this reason, the system can be used to measure accurate mass values easily, without worrying about mass calibration. In addition, the time and effort expended to date on frequent mass calibration can be reduced.
Under usual laboratory conditions, six antibiotics (with molecular weights between 265 and 1203 Da) were consecutively analyzed, and the mass error against the theoretical values was plotted. When the compounds were analyzed three at a time in positive ion mode and negative ion mode, the mass accuracy was stable with accurate mass values for all compounds to within ± 2 ppm of the theoretical values without calibrating mass even once in 72 hours.

Effortless tuning (Performance Assistant)

A sub-probe separate from the main probe is included as standard with the LCMS-9050. The ionization unit is equipped with two probes, one for analysis and one for mass calibration, so the ionization unit does not need to be replaced during mass calibration or other equipment tuning. In addition, analysis and tuning can be performed without worrying about flow line contamination. Multiple sample vials can be stored in the unit, so the system can accommodate multiple calibration samples.

The LCMS-9050 includes an auto-tuning function that uses a standard sample to automatically adjust mass accuracy, resolution, sensitivity, and other parameters.
The function includes various tuning modes that can be selected depending on the instrument status, such as the full-tuning mode for tuning the entire system or other modes for tuning only the TOF unit for mass calibration. Auto-tuning can be executed from the LabSolutions LCMS workstation software without configuring any complicated settings, which means anyone can start an analysis with the system adjusted to its optimal state.

Easy maintenance

The system inherits easy maintenance from previous models, and is designed to simplify maintenance in the vicinity of the interface. The desolvation line (DL), which injects the sample into the vacuum unit, and the ESI capillary can be replaced quickly and easily. These parts can be replaced with the vacuum maintained as is, so system downtime can be minimized.

Provides High-Speed Polarity Switching Even with TOF

New Shimadzu technology: UFstabilization

When switching between positive ion mode and negative ion mode, the flight tube voltage changes continuously to the target value. Mass errors will occur if analysis is performed before the voltage reaches the target value, so a stabilization standby time is required when switching polarity. With previous models, a certain amount of time was needed for stabilization standby, which made simultaneous positive ion/negative ion analysis difficult.
The LCMS-9050 provides high-speed polarity switching by substantially shortening the time required for stabilization standby during polarity switching. This is due to a high voltage power supply developed in-house by concentrating Shimadzu’s LCMS history and technology, and a proprietary polarity switching algorithm (UFstabilization, patent pending) developed by thoroughly investigating the properties of the high voltage power supply. Stable mass accuracy is shown even during high-speed positive/negative polarity switching, enabling simultaneous positive ion/negative ion analysis.

Stability and high mass accuracy

Just as with unipolar analysis, under usual laboratory conditions, six standard antibiotics (with molecular weights between 265 and 1203 Da) were subjected to simultaneous positive/negative ion analysis during a single analysis. The sub-interface was used to inject the external standard. Consecutive analyses were implemented until the external standard was used up, and a mass accuracy of within ±3 ppm was indicated for all compounds. Stable mass accuracy was maintained, even when performing long-term consecutive analyses using positive/negative ionization switching mode.

A world of new applications

With Q-TOF to date, when positive ion analysis and negative ion analysis were both performed, two measurements in total were required for the respective analyses, but with the LCMS-9050, the process is completed with a single measurement.
Analysis data accuracy is improved, because with pretreated samples under the same conditions, the positive/negative ions can be detected with no time difference. In addition, information on positive/negative ions can be obtained from a single analysis even for trace samples, which reduces the amount of reagents used. The data is output in a single data file, which makes it easy to compare the positive ion/negative ion analysis results.

A commercially marketed atorvastatin calcium sample was subjected to a simultaneous positive/negative ion analysis. With both positive ions and negative ions, the principle component atorvastatin and the impurities specified in the European Pharmacopeia (EP) were detected with high mass accuracy. Measuring in both positive ion and negative ion modes enables more accurate impurity identification.

Eco-friendly

The LCMS-9050 is recognized with the Eco-Products Plus label, Shimadzu’s proprietary eco-friendly product certification. Mobile phase and sample consumption can be reduced by 50 % by performing simultaneous positive ion/negative ion analysis, compared to when analyzing each mode respectively. In addition to reducing running costs during analysis, this contributes to the formation of a recycling-oriented society by promoting the efficient use of resources.

Accommodates a Variety of Needs

Diversity of optional equipment

The LCMS-9050 is designed so that a variety of optional devices can be connected. The ESI ionization unit included as standard can easily be replaced with a DPiMS probe electrospray ionization unit. In addition, the Q-TOF can be combined with the CLAM fully automatic LCMS pretreatment unit, the Nexera Mikros micro flowrate system, and the Nexera UC supercritical fluid extraction (SFE)/supercritical fluid chromatography (SFC) system.
Augmenting the basic analytical system with optional functionality broadens the world of mass spectrometry.

•  DPiMSKit for Direct Probe Ionization Mass Spectrometer
•  Nexera UCSupercritical Fluid Extraction / Supercritical Fluid Chromatograph

•  Nexera MikrosSupporting Micro Flowrate Range Liquid Chromatography Mass Spectrometry System

Convenience of PESI × LCMS-9050

PESI features quick, convenient analysis without pretreatment. Exchanging the units takes a mere 15 seconds. This uses a probe to collect and directly ionize micro sample quantities, so there is no need for investigation of LC separation conditions or complicated sample pretreatment. Fluids and solids can be measured with only simple pretreatment. Data collection is complete in less than 1 minute even when detecting both positive ions and negative ions.
Combining this with the LCMS-9050 enables the identification of compounds in samples quickly and easily.

Comprehensiveness of SFC × LCMS-9050

A feature of supercritical fluid chromatography (SFC) is its comprehensiveness, which enables the separation and analysis of a wide range of compounds varying from low polarity to high polarity. Combining this with the LCMS-9050 enables the simultaneous analysis of positive ions and negative ions, so compounds with an even wider range of properties can be batch analyzed, heightening the comprehensiveness of a single analysis.
In addition, samples extracted by supercritical fluid extraction (SFE) cannot be reused, so in ordinary Q-TOF analysis, separately extracted samples are used for positive ion measurements and negative ion measurements. With the LCMS-9050 however, positive and negative ions are measured simultaneously, so information on both positive and negative ions is obtained from samples extracted under the same conditions.

Features of PESI (Probe Electrospray Ionization)

• Perform MS analysis directly on the sample.
• Suitable for thermally labile compounds, no direct heat applied.

Measure a Variety of Samples with Only Simple Pretreatment

Rapid and Easy Drug Detection in Forensic Samples.

• Place a piece of each organ on a biological sample plate and add 50 % ethanol (35 μL).
• Detection is possible by on-plate MRM / Product Ion Scan analysis.

MT-45 Product Ion Scan Results for Each Organ

This data was obtained from joint research with Mr. Kiyotaka Usui from Tohoku University Graduate School of Medicine.

PESI MS Solution Software

PESI MS Solution software is used to control the probe, edit MS method files, and start measurements.
Scan range and SIM settings are specified via the method editing window in LabSolutions LCMS software. Analysis can be started easily by selecting the probe control mode and MS method in PESI MS Solution. During analysis, the status can be confirmed in the LabSolutions LCMS window.

Dedicated Method Package for DPiMS-8060

When using this method package with the DPiMS-8060 for mass analysis, it is no longer necessary to spend time and effort considering analysis conditions or optimizing MS parameters for each compound before starting the analysis. The simultaneous analysis of several compounds can be carried out much more efficiently.

• DPiMS-8060 Method Package for Rapid Toxicology Screening Ver. 2

Use Both Modes by Switching Easily Between ESI and PESI Units

Switch Easily Between PESI-MS and LCMS

The PESI unit can be removed easily. The ability to exchange it with an LC unit means measurements can be performed using the same theory of ionization and standard substance, which allows comparing data. The PESI unit was used for quantitative analysis of a forensic sample from a person that took the drug MT-45. As a result, we were able to obtain measurement data consistent with LCMS results in an extremely short time.

This data was obtained from joint research with Mr. Kiyotaka Usui from Tohoku University Graduate School of Medicine.

Metabolomic Analysis Performed Easily
by Transferring LCMS Analytical Conditions to DPiMS Unit

In this example, transition information for metabolites (26 components) such as amino acids, organic acids, and sugars were used for metabolomic analysis of mouse livers. Metabolomes can be analyzed easily by customizing some of the analytical conditions in the LCMS primary metabolite method package. The DPiMS-8060 system was used to measure the principal components in the model mouse group with acute liver damage induced by carbon tetrachloride and the control group. A significant difference was observed between model and control groups (given p < 0.001 for Welch’s t-test), based on the significant contribution of taurine to the separation of groups in the PCA loading plot. The difference was verified with a box-whisker plot.

This data was obtained from joint research with Associate Professor Kei Zaitsu from Nagoya University Graduate School of Medicine.

Enables Use of LC/MS/MS Method Package

The DPiMS-8060 system uses a direct analysis method that does not involve liquid chromatography using probe electrospray ionization. MRM parameters can be specified based on transition information included in the LC/MS/MS method package.
Consequently, methods can be created easily using an Excel template. A key feature of PESI is the easy process flow from pretreatment to measurement. For more detailed analysis, the ionization unit can be replaced with the ESI unit for measurements as an LC/MS/MS system.

Data Analyzed Using LabSolutions LCMS

The system can be used for a wide variety of applications, such as for library searches based on mass spectra obtained from product ion scans, for quantitative analysis based on ion chromatograms obtained from MRM, or for multivariate analysis using scan data.
Because the postrun analysis functionality in LabSolutions LCMS is used, data can also be easily compared to LC/MS/MS data.

Highly resistant to MS contamination, even with complex matrices.

Analysis System with No Carryover

Only a tiny amount of sample attaches to the probe during each sampling, reducing the risk of contamination inside the mass spectrometer to near zero.
Carryover can even be prevented by changing probes.
Even concentrated samples or samples of unknown concentration can be measured directly without pretreatment.

Mass Spectra After Repeatedly Switching Between Measuring PPGT and Blank Samples (PPGT 10 mg/mL)

3 Step Workflow

Simple pretreatment

Measure Liquid and Solid Samples with Simple Pretreatment

• Reduces the time required to analyze blood, urine, and other biological samples with conventional methods by approximately 50 %.
• Foods, tissue sections, and other solid samples can be analyzed as it. Pretreatment times are significantly reduced.

Note: Analytical precision can be improved even for solid samples by performing simple pretreatment such as dilution and centrifugation. 

Comparison of Pretreatment Times

The Functional Components of Foods Can be Detected Quickly with Simple Pretreatment

Quick and Easy Detection of Isoflavones in Processed Soy Bean Foods

MS Measurement Conditions

• Mass range: m/z 100–800 / IF Voltage: 3.5 kV
• Desolvation line temp: 250°C
• Heat blocktemp: 50°C
• Polarity:Positive / Event Time: 0.02sec

Rapid analysis

Speedy Qualitative Analysis

The qualitative analysis sequences for DPiMS QT and Q-TOF LC/MS were compared. Using DPiMS QT, it was possible to significantly reduce the measurement time in comparison to conventional methods using Q-TOF LC/MS.

Speedy Qualitative Analysis

Only a few dozen seconds are spent on DPiMS QT qualitative analysis. When performing qualitative screening analyses, the time before results are checked can be reduced, improving procedural efficiency.

High durability and stability

Analysis System with No Carryover

Only a few dozen pL of the sample adheres to the probe after each sampling. There is no worry of contamination inside the mass spectrometer. Carryover can even be prevented by changing probes. Concentrated samples and samples of unknown concentration can be measured.

Switching between DPiMS QT and Q-TOF LC/MS is Simple

Only about 15 seconds are required to remove the DPiMS QT unit, reconfiguring the system for Q-TOF LC/MS. The resources (solvents and columns) required for Q-TOF LC/MS analysis can be reduced by implementing qualitative analysis and primary screening via DPiMS QT, thereby reducing the number of samples requiring quantitative analysis.

Software

PESI MS Solution Software

PESI MS Solution software is used to control the probe, edit MS method files, and start measurements. Detailed MS analytical conditions are configured from the LabSolutions LCMS method editing window. Analysis can be started easily by selecting the probe control mode and MS method in PESI MS Solution. During analysis, the status can be confirmed in the LabSolutions LCMS window.

Sequence for Qualitative Analysis via MS and MS/MS

A qualitative analysis was performed on a whole blood sample mixed with trazodone (500 ng/mL). A cycle of MS and MS/MS measurements was performed simultaneously. LabSolutions Insight Explore was used for the data analysis. LabSolutions Insight Explore enables composition estimation, library searches, and structural analysis.
 

MS Measurement

• Checking for PeaksThe chromatogram extracted for the target is depicted by entering the composition formula or the applicable ion m/z into the compound table.
• Composition EstimationFrom the measured spectrum, select a spectrum of any m/z and use the composition estimation function to list estimated composition formulas in order by score.

MS/MS Measurement

• Assigning FragmentsUsing the structural analysis assign function in LabSolutions Insight Explore, fragments are assigned with respect to the product ion spectrum.
• Scoring via the Spectral LibraryScoring is performed with respect to the spectrum measured with DPiMS QT by creating a spectral library using Q-TOF LC/MS.

The physical properties of target compounds in metabolomics can vary greatly. An analysis method tailored to the target compounds is therefore needed for comprehensive measurements of metabolites.
This database contains multiple ready-to-use methods for comprehensive analysis of metabolites with different properties. Pretreatment examples are also included for various metabolites and samples. The user can begin a metabolomics analysis without time-consuming evaluation of LC or MS conditions.

Information for 470 compounds

This database is based on the successful LC/MS/MS Method Packages for Primary Metabolites, Cell Culture Profiling, Lipid Mediators, Short Chain Fatty Acids, and Bile Acids, and provides a comprehensive list of 470 metabolites (including internal standards) with retention times and exact mass information.

The database is compatible with the Multi-omics Analysis Package (sold separately). Processing the analysis results with Multi-omics Analysis Package allows statistical analysis, network analysis and visualization on the metabolic map(exc. Short chain fatty acids) to be carried out easily.​

*including internal standards