SFC

Feature

Supercritical Fluid Chromatography (SFC)

When a material is in a state that the temperature and the pressure exceed the critical point, it becomes a supercritical fluid. In this phase, the density of gas and liquid becomes same. Super critical fluid is a special phase which has either gas and liquid character but different from these.

• Low viscosity : Quick separation with low pressure drop
• High diffusivity : High unit productivity
• Low solvent consumption : Environmental friendly. CO₂ can be recycled.
• Acidic behavior of CO₂ : No additive such as TFA is required.

Qseries

Feature

These columns are enatioselective weak anion-exchange (AX) HPLC columns. They were developed by Prof. W. Kindber’s group in Vuena and are designed specifically for enantioselective HPLC of chiral acids and possess exceptional enantiomer separation capabilities for acidic chiral compounds containing carboxylic, phosphoric, phosphoric or sulfonic acid groups. In some cases, weakly acidic compounds such as phenols can also be separated.

CROWNPAK

Feature

Crown Ether Chiral Stationary Phases
CROWNPAK® CR(+) / CR(−) columns

These columns contain a chiral crown ether as a chiral selector which is a coated onto a 5μm silica support. Acidic mobile phases such as Perchloric acid pH 1 to 2, are used to operate these columns under standard conditions. Note that to shorten the retention time of hydrophobic samples, the addition of Methanol ( 15% maximum v/v) has been shown to be effective. These columns are the reference columns for achieving amino acid separations, with the advantage that the elution order of the enantiomers can be reversed when necessary ( CR(-) column gives the reversed elution order compared to CR(+) column).

3µm – coating type (Normal phase)

5µm – coating type (Normal phase)

10µm/20µm – coating type (Normal phase)

Feature

• Polyssacharide-based chiral stationary phases (CSPs) are most widely used for chiral separation in the world. More than 80% of enantiomers can be separated with our polysaccharide-based chiral columns.
• We prepare various size of columns from Semi-micro to Preparative type.
• We provide 3 micron series of various type of Normal Phase chiral columns.

3µm – I series

5µm – I series

Feature

Immobilized columns are designed for solvent flexibility for the resolution of compounds with limited solubility, outstanding solvent tolerance and excellent separations of enantiomers.

• High efficiency separation
• Comatible with organic solvents which give higher sample solubility as mobile phase
• More possibility for successful baseline separation
• More simple work for sample separation

CHIRALPAK® AGP

CHIRALPAK® CBH

CHIRALPAK® HSA

Feature

CHIRALPAK® AGP has extremely broad applicability for the separation of chiral drugs.

Basic compounds: CHIRALPAK® AGP、CHIRALPAK® CBHAcids, Non-protolytic compounds: CHIRALPAK® AGP、CHIRALPAK® HSA

• CHIRALPAK® AGP has extremely broad applicability for the separation of chiral drugs.
• The protein-based columns function entirely in the reversed-phase chromatographic mode, using buffers with low organic modifier content and at moderate pH values.
• Possibility to dynamically improve enantioseparation.

CHIRALPAK AGP® has extremely broad applicability for the separation of chiral drugs.

○The chiral selector is alpha1-acid glycoprotein (AGP). This very stable protein is immobilized onto spherical 5 micrometer silica particles.
The CHIRALPAK® AGP column separates enantiomers of an extremely broad range of drug compounds.

・Amine (primary, secondary, and tertiary)
・Acids (strong and weak)
・Nonprotolytes (amides, esters, alchohols, sulfoxides, etc.)

CHIRALPAK CBH

○The chiral selector is Cellobiohydrolase (CBH). This is a stable enzyme, which has been immobilized onto 5 micrometer spherical silica particles.

○CHIRALPAK® CBH is used for the separation of enantiomers of basic drugs from many compound classes. CHIRALPAK® CBH separates preferentially compounds containing one or more basic nitrogen’s together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.).

CHIRALPAK HSA

○The chiral selector is human serum albumin (HSA). The protein has been immobilized onto spherical 5 micrometer particles.

○CHIRALPAK® HSA is especially suited for the separation of weak and strong acids, zwitterionic and nonprotolytic compounds.

3µm – coating type (Reverse phase)

5µm – coating type (Reverse phase)

10µm – coating type (Reverse phase)

Feature

• For reverse phase use
• You can analyze the optical purity of metabolic substance and sample which is produced by bio-method, under water-based mobile phase condition.
• Available for LC/MS (we prepare 2.1mm ID products.)
• 3 micron type is available

Guard Cartridge Holder

Jacket

Feature

Guard Cartridge Holder

The HPLC analytical guard cartridges need to be mounted in a cartridge holder to be connected to the analytical column. For maximum lifetime, analytical columns should be used in series with an appropriate guard cartridge or column. Both formats are available with the packing material to match the protected column.Available HPLC Analytical Guard Cartridges for Use with Cartridge HolderCHIRALPAK® IA-3, CHIRALPAK® IA, CHIRALPAK® IB-3, CHIRALPAK® IB, CHIRALPAK® IC-3, CHIRALPAK® IC, CHIRALPAK® ID-3, CHIRALPAK® ID, CHIRALPAK® IE-3, CHIRALPAK® IE, CHIRALPAK® IF-3, CHIRALPAK® IF, CHIRALPAK® AD-3, CHIRALPAK® AD-H, CHIRALPAK® AS-3, CHIRALPAK® AS-H, CHIRALPAK® AY-3, CHIRALPAK® AY-H, CHIRALPAK® AZ-3, CHIRALPAK® AZ-H, CHIRALCEL® OD-3, CHIRALCEL® OD-H, CHIRALCEL® OJ-3, CHIRALCEL® OJ-H, CHIRALCEL® OZ-3, CHIRALCEL® OZ-H, CHIRALCEL® OX-3, CHIRALCEL® OX-H, CHIRALPAK® AD-3R, CHIRALPAK® AD-RH, CHIRALPAK® AS-3R, CHIRALPAK® AS-RH, CHIRALPAK® AY-3R, CHIRALPAK® AY-RH,CHIRALPAK® AZ-3R, CHIRALPAK® AZ-RH, CHIRALCEL® OD-3R, CHIRALCEL® OD-RH, CHIRALCEL® OD-R, CHIRALCEL® OJ-3R, CHIRALCEL® OJ-RH, CHIRALCEL® OZ-3R, CHIRALCEL® OZ-RH, CHIRALCEL® OB-H, CHIRALCEL® OC-H, CHIRALPAK® QN-AX, CHIRALPAK® QD-AX

•  We prepare Guard columns (Analytical & Semi-prep) for CHIRALPAK® AD/AS/OP (+) /OT (+) , CHIRALCEL® OA/OB/OC/OD/OF/OG/OJ/OK.

Column jacket

Column jackets allow operating temperatures of analytical columns to be controlled with a circulating water bath.

Other

Feature

Ligand Exchange Chiral Stationary Phases
CHIRALPAK® WH / CHIRALPAK® MA(+) columns

The chiral stationary phases in these columns are made of amino acids and its derivatives coated or bonded to silica supports ( with a particle size of 10μm for WH and 3μm for MA (+) ) . Since these columns are ligand-exchange type columns, the standard mobile phase to use is an aqueous solution of CuSO4 (0.1 to 2mM) . These columns can tolerate organic moditifiers such as Methanol and Acetonitrile according to the specifications in the instruction manual.

Polymethacrylate Chiral Stationary Phases
CHIRALPAK® OP(+) / CHIRALPAK® OT(+) columns

The chiral selector is a chiral synthetic methacrylate polymer coated onto a 10μm silica suppor t. The best chromatographic results are obtained using 100% Methanol as mobile phase. The polymer used for the CHIRALPAK OT (+) column is very delicate and is slowly degraded by alcohols. To avoid this phenomenon, we recommend to run the analyses at low temperatures ( 0~5 ).