Striving for the Ultimate in Accuracy and Easy Operability

By combining new technologies with those cultivated over Shimadzu’s long history, the Shimadzu spectrofluorophotometer has been reborn as the RF-6000. Combined with new LabSolutions RF software, designed for unrivaled measurement accuracy and easy operation, the RF-6000 offers the ultimate performance for a diverse range of customers’ measurement needs.

Wide Variety of Spectral Techniques

  • Enhanced sensitivity and dynamic range enable fluorescence as well as bioluminescence, chemiluminescence, and electro-luminescence measurements.
  • High-speed 3D scanning enables rapid acquisition of 3D spectra.
  • Spectral-Corrected Excitation and Emission spectra can be scanned.
  • Fluorescence quantum yield and Fluorescence quantum efficiency measurements are available.

High Sensitivity, High Stability and High Speed

  • Highest level SNR in its class: 1,000 or more (RMS) /350 or more (P-P)
  • High-speed scanning of 60,000 nm/min minimizes scan time.
  • 2,000 hour long life Xe lamp.
  • Extended range PMT offers scan wavelength range to 900nm.

Excellent Usability

  • Easy-to-use LabSolutions RF software simplifies analysis.
  • Validation routines included.
  • Status bar in LabSolutions RF indicates lamp and accessory status.
  • Large sample compartment for all analytical needs.

Wide Variety of Spectral Techniques

Supports Applications in a Wide Variety of Fields

High Sensitivity, High Stability and High Speed

■ High-sensitivity measurements can be performed with S/N ratios of 1,000:1 (RMS) or 350:1 (P-P).
■ Improved lower limit of quantitation. Measures concentrations up to 1 x 10-13 mol/L (fluorescein).

A redesigned optical system and signal processing system achieve the highest S/N ratio levels in its class.
Even extremely dilute samples can be measured accurately.

The RF-6000 can measure ­uorescence spectra from ­uorescein concentrations as low as 1 x 10-13 mol/L. Furthermore, due to an auto-gain control function that ensures measurements are performed using optimal measurement parameters, the system can perform accurate and highly quantitative measurements over a wide six-order dynamic range, from 10-13 to 10-7 mol/L.

Enables ultrafast scanning at speeds up to 60,000 nm/min. All wavelength regions can be measured in only one second. 3D fluorescence spectra can also be measured quickly.

The Xenon lamp offers long service life and high stability. The 2000-hour service life significantly reduces running costs. In addition, auto alignment technology allows customers to easily replace the lamp without tedious adjustment routines.

A low-noise photomultiplier is included standard. It offers high measurement sensitivity for long wavelengths up to 900 nm. Consequently, it can measure substances that exhibit fluorescence at longer wavelengths, such as chlorophyll and indocyanin green (ICG). Below are Excitation and Emission spectra of indocyanin green, which is used for testing hepatic function and hepatic spare ability. The fluorescence peak was at 810 nm.

Fluorescence Spectrum of Thylakoid membrene solution Cooled by Liquid Nitrogen

*1:Measured with the help of professor Jian-Ren Shen of Okayama University.
*2:Measured using a low-temperature measurement unit. Contact Shimadzu for further details about the low-temperature measurement unit.


LabTube Meets Mark Talbott, Shimadzu

LabTube Meets Mark Talbott, Shimadzu at Pittcon 2015, New Orleans. In this video Mark introduces Shimadzu’s new RF-6000 fluorescence spectrophotometer.

How to register a new spectrometer, how to collect a 2D spectrum and how to collect a 3D spectrum.

Included in this video are how to register a new spectrometer, how to collect a 2D spectrum and how to collect a 3D spectrum.


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